Detailed information of TA system TA166018

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1084950..1085571
• Replicon: chromosome
• Accession: NZ_CP058172
• Organism: Enterobacter hormaechei strain RHBSTW-00056

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A837FFM2
• Locus tag: HV047_RS05250
• Protein ID: WP_015571250.1
• Coordinates: 1084950..1085168 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: HV047_RS05255
• Protein ID: WP_155025759.1
• Coordinates: 1085197..1085571 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV047_RS05220	1080962..1081222	+	261	WP_015571255.1	type B 50S ribosomal protein L31	-
HV047_RS05225	1081225..1081365	+	141	WP_003859006.1	type B 50S ribosomal protein L36	-
HV047_RS05230	1081362..1082072	-	711	WP_155025761.1	GNAT family N-acetyltransferase	-
HV047_RS05235	1082175..1083635	+	1461	WP_155025760.1	PLP-dependent aminotransferase family protein	-
HV047_RS05240	1083607..1084074	-	468	WP_022647269.1	hypothetical protein	-
HV047_RS05245	1084191..1084742	-	552	WP_022647270.1	maltose O-acetyltransferase	-
HV047_RS05250	1084950..1085168	-	219	WP_015571250.1	hemolysin expression modulator Hha	Toxin
HV047_RS05255	1085197..1085571	-	375	WP_155025759.1	Hha toxicity modulator TomB	Antitoxin
HV047_RS05260	1086081..1089227	-	3147	WP_023299464.1	multidrug efflux RND transporter permease subunit	-
HV047_RS05265	1089250..1090443	-	1194	WP_022647272.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8583.95 Da
• Isoelectric Point: 8.9008

>T166018 WP_015571250.1 NZ_CP058172:c1085168-1084950 [Enterobacter hormaechei]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Nucleotide

• Length: 219 bp

>T166018 NZ_CP074019:4757683-4757994 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14481.32 Da
• Isoelectric Point: 5.1542

>AT166018 WP_155025759.1 NZ_CP058172:c1085571-1085197 [Enterobacter hormaechei]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
VDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Nucleotide

• Length: 375 bp

>AT166018 NZ_CP074019:4757995-4758285 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A837FFM2