Detailed information of TA system TA166015

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 56393..57036
• Replicon: plasmid pRHBSTW-00059_2
• Accession: NZ_CP058168
• Organism: Enterobacter hormaechei strain RHBSTW-00059

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HV048_RS22875
• Protein ID: WP_057072957.1
• Coordinates: 56393..56809 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: HV048_RS22880
• Protein ID: WP_057072956.1
• Coordinates: 56806..57036 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV048_RS22840	52539..53009	-	471	WP_004883378.1	RES family NAD+ phosphorylase	-
HV048_RS22845	53006..53449	-	444	WP_032413443.1	DUF2384 domain-containing protein	-
HV048_RS22850	53550..53779	-	230	Protein_54	DUF4113 domain-containing protein	-
HV048_RS22855	53820..54137	+	318	Protein_55	transposase	-
HV048_RS22860	54351..54770	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	-
HV048_RS22865	54767..55078	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	-
HV048_RS22870	55283..56343	-	1061	Protein_58	IS3 family transposase	-
HV048_RS22875	56393..56809	-	417	WP_057072957.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV048_RS22880	56806..57036	-	231	WP_057072956.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HV048_RS22885	57636..57854	+	219	WP_016240352.1	type II toxin-antitoxin system antitoxin CcdA	-
HV048_RS22890	57856..58161	+	306	WP_045616569.1	type II toxin-antitoxin system toxin CcdB	-
HV048_RS22895	58331..59347	+	1017	WP_063216424.1	hypothetical protein	-
HV048_RS22900	59545..60339	+	795	WP_016240356.1	site-specific integrase	-
HV048_RS22905	60550..61275	-	726	Protein_65	IS5-like element ISKpn26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ	1..77568	77568
	-	inside	IScluster/Tn	-	-	51221..63946	12725

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15051.55 Da
• Isoelectric Point: 7.8629

>T166015 WP_057072957.1 NZ_CP058168:c56809-56393 [Enterobacter hormaechei]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRNQRIVVSAITYSEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDAATDIKVALRLAGTPIGPNDTLIAGHAIAAGAVLVTRDGGEFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T166015 NZ_CP074019:c3709651-3709244 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8797.88 Da
• Isoelectric Point: 4.3654

>AT166015 WP_057072956.1 NZ_CP058168:c57036-56806 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFDGVSQLEIVREGDSIILRPVRPTWGSFFELEKADPDFMAEREDVVTDEGRFEP

Nucleotide

• Length: 231 bp

>AT166015 NZ_CP074019:c3709898-3709632 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA