Detailed information of TA system TA166000

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 96850..97569
• Replicon: chromosome
• Accession: NZ_CP058167
• Organism: Enterobacter hormaechei strain RHBSTW-00059

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A144XIR2
• Locus tag: HV048_RS00490
• Protein ID: WP_023305176.1
• Coordinates: 96850..97170 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HV048_RS00495
• Protein ID: WP_032660963.1
• Coordinates: 97204..97569 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV048_RS00455	92743..93531	+	789	WP_115168434.1	winged helix-turn-helix domain-containing protein	-
HV048_RS00460	93533..94012	+	480	WP_022646606.1	hypothetical protein	-
HV048_RS00465	94255..95136	+	882	WP_032670896.1	helix-turn-helix domain-containing protein	-
HV048_RS00470	95201..95500	-	300	WP_023299004.1	XRE family transcriptional regulator	-
HV048_RS00475	95624..95965	-	342	WP_023305175.1	SymE family type I addiction module toxin	-
HV048_RS00480	96093..96254	+	162	WP_065212817.1	DUF3950 domain-containing protein	-
HV048_RS00485	96367..96573	-	207	WP_071788007.1	hypothetical protein	-
HV048_RS00490	96850..97170	-	321	WP_023305176.1	TA system toxin CbtA family protein	Toxin
HV048_RS00495	97204..97569	-	366	WP_032660963.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV048_RS00500	97601..97822	-	222	WP_023305178.1	DUF987 family protein	-
HV048_RS00505	97831..98301	-	471	WP_023305179.1	DNA repair protein RadC	-
HV048_RS00510	98371..99192	-	822	WP_023305180.1	DUF945 domain-containing protein	-
HV048_RS00515	99885..100724	-	840	WP_050582604.1	hypothetical protein	-
HV048_RS00520	100837..101271	-	435	WP_023305183.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	92743..119290	26547

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (5-104)

Antitoxin

• Antitoxin CbeA (18-116)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12190.77 Da
• Isoelectric Point: 5.9559

>T166000 WP_023305176.1 NZ_CP058167:c97170-96850 [Enterobacter hormaechei]
MHISTVPATVTVSSRLSPVQVWQKLLTYILEHHYGLTINDTPFSNDTTIQEHIEAGVNLTDAVNFLVERFELVRIDQKGF
SWQDQEPWITSLDVHRAQFNLGLKRS

Nucleotide

• Length: 321 bp

>T166000 NZ_CP074019:c351621-351244 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13456.20 Da
• Isoelectric Point: 6.8421

>AT166000 WP_032660963.1 NZ_CP058167:c97569-97204 [Enterobacter hormaechei]
MQSTTLSGTSAENASCLQWGLKRNITPCFGARLVQEGNRLYFLADRAGFNGSFTDDEALRLDQAFPLMMKQLERMLTAGE
LDPRSQHCVTRHHNGLTCEADTLGSHGYVYIAIYPQSALTR

Nucleotide

• Length: 366 bp

>AT166000 NZ_CP074019:c352042-351668 [Escherichia coli]
GTGTCAGACACACACCCCGGGACAACGCATCCCGACAACAACAACGACCGCCCCTGGTGGGGGCTCCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGGCAGT
TTAGCGACGCGGATGCGTATCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A144XIR2