Detailed information of TA system TA165982

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 71220..71746
• Replicon: plasmid pRHBSTW-00083_2
• Accession: NZ_CP058161
• Organism: Enterobacter hormaechei strain RHBSTW-00083

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: HV059_RS23065
• Protein ID: WP_000323025.1
• Coordinates: 71459..71746 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: J5W3H0
• Locus tag: HV059_RS23060
• Protein ID: WP_004196370.1
• Coordinates: 71220..71459 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV059_RS23030	66557..66749	-	193	Protein_81	MFS transporter	-
HV059_RS23035	66779..67756	+	978	WP_004196334.1	chromate resistance protein	-
HV059_RS23040	67713..69089	+	1377	WP_004196363.1	chromate efflux transporter	-
HV059_RS23045	69120..69809	-	690	WP_004196322.1	hypothetical protein	-
HV059_RS23050	69823..70560	-	738	WP_008460272.1	HupE/UreJ family protein	-
HV059_RS23055	70604..70969	-	366	WP_009651956.1	hypothetical protein	-
HV059_RS23060	71220..71459	+	240	WP_004196370.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
HV059_RS23065	71459..71746	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
HV059_RS23070	71818..71976	+	159	WP_013087178.1	type I toxin-antitoxin system Hok family toxin	-
HV059_RS23075	72855..73169	+	315	WP_023338051.1	hypothetical protein	-
HV059_RS23080	73229..73690	+	462	WP_048974901.1	hypothetical protein	-
HV059_RS23085	73780..73980	+	201	WP_031494497.1	hypothetical protein	-
HV059_RS23090	74021..74812	+	792	WP_032663722.1	N-6 DNA methylase	-
HV059_RS23095	74855..75190	+	336	WP_023345479.1	hypothetical protein	-
HV059_RS23100	75880..76173	+	294	WP_032663725.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..113845	113845

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (3-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T165982 WP_000323025.1 NZ_CP058161:71459-71746 [Enterobacter hormaechei]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T165982 NZ_CP074012:798523-798819 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9018.43 Da
• Isoelectric Point: 4.3894

>AT165982 WP_004196370.1 NZ_CP058161:71220-71459 [Enterobacter hormaechei]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLCNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT165982 NZ_CP074012:798821-799216 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• AlphaFold DB: A0A2J4QRH4