Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 24736..25379 Replicon plasmid pRHBSTW-00083_2
Accession NZ_CP058161
Organism Enterobacter hormaechei strain RHBSTW-00083

Toxin (Protein)


Gene name vagD Uniprot ID A0A155IU92
Locus tag HV059_RS22775 Protein ID WP_000754567.1
Coordinates 24736..25152 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A155IUD1
Locus tag HV059_RS22780 Protein ID WP_023293777.1
Coordinates 25149..25379 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV059_RS22755 21915..22484 - 570 WP_023332907.1 small heat shock protein sHSP20 -
HV059_RS22760 22519..22800 - 282 WP_023332908.1 helix-turn-helix domain-containing protein -
HV059_RS22765 22994..23257 - 264 WP_001295708.1 hypothetical protein -
HV059_RS22770 23417..24385 + 969 WP_016151349.1 IS5 family transposase -
HV059_RS22775 24736..25152 - 417 WP_000754567.1 type II toxin-antitoxin system VapC family toxin Toxin
HV059_RS22780 25149..25379 - 231 WP_023293777.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HV059_RS22785 25688..26554 + 867 WP_050596932.1 hypothetical protein -
HV059_RS22790 26666..26851 + 186 WP_032656023.1 hypothetical protein -
HV059_RS22795 26901..28055 - 1155 WP_181238482.1 hypothetical protein -
HV059_RS22800 28456..28956 + 501 WP_032673389.1 hypothetical protein -
HV059_RS22805 28969..29748 + 780 WP_032673387.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..113845 113845
- flank IS/Tn - - 23417..24385 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15108.61 Da        Isoelectric Point: 8.5403

>T165981 WP_000754567.1 NZ_CP058161:c25152-24736 [Enterobacter hormaechei]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T165981 NZ_CP074012:749800-750177 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8706.73 Da        Isoelectric Point: 4.4299

>AT165981 WP_023293777.1 NZ_CP058161:c25379-25149 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAQREDVVSDEGRFDP

Download         Length: 231 bp

>AT165981 NZ_CP074012:749342-749710 [Escherichia coli]
GTGTCAGACACATTCTCCGGGACAACGCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGTACCGTGAC
ACCTTGTTTTGGCGCCCGTCTGGTGCAGGAGGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATTCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A155IU92

Antitoxin

Source ID Structure
AlphaFold DB A0A155IUD1

References