Detailed information of TA system TA165957

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 31638..32275
• Replicon: plasmid pRHBSTW-00086_2
• Accession: NZ_CP058158
• Organism: Enterobacter hormaechei strain RHBSTW-00086

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A9E6WXZ4
• Locus tag: HV061_RS23095
• Protein ID: WP_032662169.1
• Coordinates: 31638..32048 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A9E6WTF5
• Locus tag: HV061_RS23100
• Protein ID: WP_032662167.1
• Coordinates: 32045..32275 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV061_RS23075	27003..27353	+	351	WP_032662182.1	DUF305 domain-containing protein	-
HV061_RS23080	27539..28447	-	909	WP_032662179.1	HNH endonuclease	-
HV061_RS23085	28995..30017	-	1023	WP_040119796.1	helicase UvrD	-
HV061_RS23090	30002..31564	-	1563	WP_040119797.1	AAA family ATPase	-
HV061_RS23095	31638..32048	-	411	WP_032662169.1	PIN domain-containing protein	Toxin
HV061_RS23100	32045..32275	-	231	WP_032662167.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HV061_RS23105	32621..35746	+	3126	WP_063201741.1	hypothetical protein	-
HV061_RS23110	36033..36323	+	291	WP_040119769.1	hypothetical protein	-
HV061_RS23115	36313..37212	+	900	WP_040119770.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..113610	113610

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-127)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14729.03 Da
• Isoelectric Point: 5.7351

>T165957 WP_032662169.1 NZ_CP058158:c32048-31638 [Enterobacter hormaechei]
VNKMYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITWAEVSLASQEAGPAAQKLADAFAARLDAILPWDRTAV
DATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVR

Nucleotide

• Length: 411 bp

>T165957 NZ_CP074007:c910318-909854 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8645.77 Da
• Isoelectric Point: 4.4692

>AT165957 WP_032662167.1 NZ_CP058158:c32275-32045 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMAERGDVVSDEGRVNL

Nucleotide

• Length: 231 bp

>AT165957 NZ_CP074007:c910653-910318 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA