Detailed information of TA system TA165955

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3802153..3802832
• Replicon: chromosome
• Accession: NZ_CP058157
• Organism: Enterobacter hormaechei strain RHBSTW-00086

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: HV061_RS18205
• Protein ID: WP_000854680.1
• Coordinates: 3802491..3802832 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: HV061_RS18200
• Protein ID: WP_000070396.1
• Coordinates: 3802153..3802470 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV061_RS18155	3797591..3798412	+	822	WP_000197389.1	DUF945 domain-containing protein	-
HV061_RS18160	3798629..3799330	+	702	WP_001581462.1	WYL domain-containing protein	-
HV061_RS18165	3799371..3799607	+	237	WP_001144031.1	hypothetical protein	-
HV061_RS18170	3799607..3800050	+	444	WP_121909192.1	hypothetical protein	-
HV061_RS18175	3800073..3800540	+	468	WP_001547765.1	hypothetical protein	-
HV061_RS18180	3800617..3800856	+	240	WP_000194654.1	DUF905 domain-containing protein	-
HV061_RS18185	3800954..3801412	+	459	WP_063448507.1	antirestriction protein	-
HV061_RS18190	3801428..3801904	+	477	WP_000811693.1	RadC family protein	-
HV061_RS18195	3801913..3802134	+	222	WP_000691994.1	DUF987 domain-containing protein	-
HV061_RS18200	3802153..3802470	+	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
HV061_RS18205	3802491..3802832	+	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
HV061_RS18215	3803401..3804654	-	1254	WP_063202005.1	glutamate-5-semialdehyde dehydrogenase	-
HV061_RS18220	3804666..3805769	-	1104	WP_015571369.1	glutamate 5-kinase	-
HV061_RS18225	3806075..3807127	+	1053	WP_022647150.1	phosphoporin PhoE	-
HV061_RS18230	3807259..3807660	-	402	WP_022647149.1	sigma factor-binding protein Crl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T165955 WP_000854680.1 NZ_CP058157:3802491-3802832 [Enterobacter hormaechei]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T165955 NZ_CP074005:c4227401-4227228 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT165955 WP_000070396.1 NZ_CP058157:3802153-3802470 [Enterobacter hormaechei]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT165955 NZ_CP074005:c4227725-4227429 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7