Detailed information of TA system TA165953

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2371685..2372424
• Replicon: chromosome
• Accession: NZ_CP058157
• Organism: Enterobacter hormaechei strain RHBSTW-00086

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HV061_RS11235
• Protein ID: WP_063139083.1
• Coordinates: 2371939..2372424 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A837FCR9
• Locus tag: HV061_RS11230
• Protein ID: WP_003857131.1
• Coordinates: 2371685..2371951 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV061_RS11205	2366833..2367357	+	525	WP_087878309.1	fimbrial protein	-
HV061_RS11210	2367381..2368517	+	1137	WP_048966029.1	type 1 fimbrial protein	-
HV061_RS11215	2368638..2369195	+	558	WP_045622668.1	OmpH family outer membrane protein	-
HV061_RS11220	2369174..2370562	-	1389	WP_148741973.1	MFS transporter	-
HV061_RS11225	2370692..2371621	+	930	WP_063200243.1	LysR family transcriptional regulator	-
HV061_RS11230	2371685..2371951	+	267	WP_003857131.1	DUF1778 domain-containing protein	Antitoxin
HV061_RS11235	2371939..2372424	+	486	WP_063139083.1	GNAT family N-acetyltransferase	Toxin
HV061_RS11240	2372450..2372770	-	321	Protein_2192	hypothetical protein	-
HV061_RS11245	2372782..2373111	+	330	WP_148741971.1	hypothetical protein	-
HV061_RS11250	2373086..2373366	-	281	Protein_2194	DUF4225 domain-containing protein	-
HV061_RS11255	2374294..2375736	+	1443	WP_080454133.1	ISNCY family transposase	-
HV061_RS11260	2375860..2376504	-	645	WP_148741969.1	CPBP family intramembrane metalloprotease	-
HV061_RS11265	2377002..2377334	-	333	WP_023300105.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2374336..2375736	1400

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (58-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17544.22 Da
• Isoelectric Point: 9.6275

>T165953 WP_063139083.1 NZ_CP058157:2371939-2372424 [Enterobacter hormaechei]
VGRVTPPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSIRGNGLGADLLQDAVLRCYRVAENIGVRAIMVHALTEDAKAFYIHHGFKASQTQERTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T165953 NZ_CP074005:c3671867-3671469 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.50 Da
• Isoelectric Point: 4.3837

>AT165953 WP_003857131.1 NZ_CP058157:2371685-2371951 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT165953 NZ_CP074005:c3672163-3671870 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A837FCR9