Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 1680859..1681656 Replicon chromosome
Accession NZ_CP058153
Organism Enterobacter hormaechei strain RHBSTW-00105

Toxin (Protein)


Gene name KacT Uniprot ID A0A4Q2R3N2
Locus tag HV071_RS07920 Protein ID WP_032670168.1
Coordinates 1681135..1681656 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID F5RT92
Locus tag HV071_RS07915 Protein ID WP_001303459.1
Coordinates 1680859..1681128 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV071_RS07885 1676399..1676758 + 360 WP_040117469.1 helix-turn-helix transcriptional regulator -
HV071_RS07890 1677084..1678325 + 1242 WP_047051685.1 bifunctional glucose-1-phosphatase/inositol phosphatase -
HV071_RS07895 1678358..1678585 - 228 WP_006809324.1 YccJ family protein -
HV071_RS07900 1678604..1679200 - 597 WP_006809323.1 NAD(P)H:quinone oxidoreductase -
HV071_RS07905 1679592..1679762 + 171 WP_001273664.1 general stress protein -
HV071_RS07910 1679879..1680784 + 906 WP_047051684.1 DMT family transporter -
HV071_RS07915 1680859..1681128 + 270 WP_001303459.1 DUF1778 domain-containing protein Antitoxin
HV071_RS07920 1681135..1681656 + 522 WP_032670168.1 GNAT family N-acetyltransferase Toxin
HV071_RS07925 1681715..1683037 - 1323 WP_048982825.1 pyrimidine utilization transport protein G -
HV071_RS07930 1683059..1683550 - 492 WP_047052688.1 pyrimidine utilization flavin reductase protein F -
HV071_RS07935 1683563..1684153 - 591 WP_047052687.1 malonic semialdehyde reductase -
HV071_RS07940 1684163..1684963 - 801 WP_047052686.1 pyrimidine utilization protein D -
HV071_RS07945 1684971..1685357 - 387 WP_032670161.1 pyrimidine utilization protein C -
HV071_RS07950 1685369..1686058 - 690 WP_047052685.1 pyrimidine utilization protein B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19437.18 Da        Isoelectric Point: 7.4318

>T165925 WP_032670168.1 NZ_CP058153:1681135-1681656 [Enterobacter hormaechei]
VANLTIEMLSEGTDYDFGDFDCGEPSLNAFLTEHLVRQHGGRILRGYLLKERDHPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQA

Download         Length: 522 bp

>T165925 NZ_CP074001:1770431-1770685 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CATTGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10248.70 Da        Isoelectric Point: 5.8836

>AT165925 WP_001303459.1 NZ_CP058153:1680859-1681128 [Enterobacter hormaechei]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Download         Length: 270 bp

>AT165925 NZ_CP074001:1770183-1770434 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGCCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4Q2R3N2

Antitoxin

Source ID Structure
AlphaFold DB A0A7D7BCZ2

References