Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5036..5561 Replicon plasmid pRHBSTW-00114_3
Accession NZ_CP058151
Organism Enterobacter hormaechei strain RHBSTW-00114

Toxin (Protein)


Gene name ccdB Uniprot ID A0A1D8K3Q5
Locus tag HV076_RS23700 Protein ID WP_006788214.1
Coordinates 5036..5341 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag HV076_RS23705 Protein ID WP_006788213.1
Coordinates 5343..5561 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV076_RS23675 1479..2105 + 627 WP_016946792.1 ParA family plasmid-partitioning AAA ATPase -
HV076_RS23680 2102..2404 + 303 WP_181236891.1 hypothetical protein -
HV076_RS23685 2552..2749 + 198 WP_181236893.1 hypothetical protein -
HV076_RS23690 2836..3630 - 795 WP_016240356.1 site-specific integrase -
HV076_RS23695 3866..4351 - 486 Protein_5 hypothetical protein -
HV076_RS23700 5036..5341 - 306 WP_006788214.1 type II toxin-antitoxin system toxin CcdB Toxin
HV076_RS23705 5343..5561 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HV076_RS23710 5731..5976 - 246 Protein_8 hypothetical protein -
HV076_RS23715 6077..6418 + 342 WP_152967296.1 hypothetical protein -
HV076_RS23720 6500..7957 - 1458 WP_057072997.1 amidohydrolase -
HV076_RS23725 8067..8933 - 867 WP_057072971.1 transporter substrate-binding domain-containing protein -
HV076_RS23730 9014..9778 - 765 WP_057072961.1 ATP-binding cassette domain-containing protein -
HV076_RS23735 9775..10503 - 729 WP_057072996.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - mrkA / mrkB / mrkC / mrkD / mrkF 1..84905 84905


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11687.46 Da        Isoelectric Point: 7.2015

>T165916 WP_006788214.1 NZ_CP058151:c5341-5036 [Enterobacter hormaechei]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRLMTTDMASVPASVTGEEV
ADLRHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T165916 NZ_CP073996:c4572920-4572534 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT165916 WP_006788213.1 NZ_CP058151:c5561-5343 [Enterobacter hormaechei]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT165916 NZ_CP073996:c4572544-4572215 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCATTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D8K3Q5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References