Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 4194801..4195480 Replicon chromosome
Accession NZ_CP058146
Organism Enterobacter hormaechei strain RHBSTW-00133

Toxin (Protein)


Gene name ykfI Uniprot ID R8WLP2
Locus tag HV088_RS20230 Protein ID WP_006812012.1
Coordinates 4194801..4195142 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID R8WMM2
Locus tag HV088_RS20235 Protein ID WP_006812013.1
Coordinates 4195163..4195480 (-) Length 106 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV088_RS20185 4190009..4190236 - 228 WP_032634595.1 TraR/DksA family transcriptional regulator -
HV088_RS20190 4190236..4190469 - 234 WP_181236547.1 DUF2732 domain-containing protein -
HV088_RS20195 4190537..4190878 - 342 WP_032610572.1 DUF5347 domain-containing protein -
HV088_RS20200 4190842..4191042 - 201 WP_015386352.1 DUF2724 domain-containing protein -
HV088_RS20205 4191050..4191559 - 510 WP_015386351.1 phage regulatory CII family protein -
HV088_RS20210 4191594..4191830 - 237 WP_015386350.1 hypothetical protein -
HV088_RS20215 4191932..4192546 + 615 WP_032610574.1 phage repressor protein CI -
HV088_RS20220 4192558..4193457 + 900 WP_032610575.1 NAD-dependent DNA ligase -
HV088_RS20225 4193467..4194480 + 1014 WP_032610576.1 tyrosine-type recombinase/integrase -
HV088_RS20230 4194801..4195142 - 342 WP_006812012.1 TA system toxin CbtA family protein Toxin
HV088_RS20235 4195163..4195480 - 318 WP_006812013.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HV088_RS20240 4195500..4195721 - 222 WP_000691995.1 DUF987 domain-containing protein -
HV088_RS20245 4195730..4196206 - 477 WP_047355118.1 RadC family protein -
HV088_RS20250 4196222..4196680 - 459 WP_112020495.1 antirestriction protein -
HV088_RS20255 4196783..4197022 - 240 WP_112020496.1 DUF905 domain-containing protein -
HV088_RS20260 4197099..4197566 - 468 WP_063841896.1 hypothetical protein -
HV088_RS20265 4197589..4198032 - 444 WP_001547764.1 phage transcriptional regulator -
HV088_RS20270 4198032..4198268 - 237 WP_001115854.1 hypothetical protein -
HV088_RS20275 4198305..4199006 - 702 WP_008324530.1 WYL domain-containing protein -
HV088_RS20280 4199224..4200045 - 822 WP_097535357.1 DUF945 domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4156623..4200045 43422


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(5-104)


Sequences


Toxin        


Download         Length: 114 a.a.        Molecular weight: 12989.07 Da        Isoelectric Point: 10.4476

>T165899 WP_006812012.1 NZ_CP058146:c4195142-4194801 [Enterobacter hormaechei]
MKTLPATTQRAAKPCLAPVAVWQMLLTRLLKQHYGLTLNDTPFSEERVIQEHIDAGITLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRKNSVR

Download         Length: 342 bp

>T165899 NZ_CP073996:c984275-984172 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11874.45 Da        Isoelectric Point: 7.0166

>AT165899 WP_006812013.1 NZ_CP058146:c4195480-4195163 [Enterobacter hormaechei]
MTHINWGLQRAITPRLGARLVQEGNRLHYLADRASITGRFSDTECRKLDETCPHFIRQMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQS

Download         Length: 318 bp

>AT165899 NZ_CP073996:984134-984405 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB R8WLP2


Antitoxin

Source ID Structure
AlphaFold DB R8WMM2

References