Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 82066..82802 Replicon plasmid pRHBSTW-00138_2
Accession NZ_CP058132
Organism Klebsiella quasipneumoniae strain RHBSTW-00138

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag HV092_RS26475 Protein ID WP_003026803.1
Coordinates 82320..82802 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag HV092_RS26470 Protein ID WP_003026799.1
Coordinates 82066..82332 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV092_RS26435 77910..78272 - 363 WP_004206665.1 arsenite efflux transporter metallochaperone ArsD -
HV092_RS26440 78324..78674 - 351 WP_004206664.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
HV092_RS26445 79032..79280 + 249 WP_004193994.1 hypothetical protein -
HV092_RS26450 79277..80488 + 1212 WP_032415728.1 hypothetical protein -
HV092_RS26455 80622..81113 + 492 WP_004206662.1 hypothetical protein -
HV092_RS26460 81174..81377 + 204 WP_004206661.1 hemolysin expression modulator Hha -
HV092_RS26465 81391..81621 + 231 WP_004206660.1 hypothetical protein -
HV092_RS26470 82066..82332 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
HV092_RS26475 82320..82802 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
HV092_RS26480 83004..84407 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
HV092_RS26485 84436..85068 - 633 WP_001567369.1 hypothetical protein -
HV092_RS26490 85245..86777 - 1533 WP_181352630.1 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA 1..199458 199458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T165884 WP_003026803.1 NZ_CP058132:82320-82802 [Klebsiella quasipneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T165884 NZ_CP073994:2069331-2069434 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT165884 WP_003026799.1 NZ_CP058132:82066-82332 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT165884 NZ_CP073994:c2069472-2069201 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References