Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 16859..17502 Replicon plasmid pRHBSTW-00138_2
Accession NZ_CP058132
Organism Klebsiella quasipneumoniae strain RHBSTW-00138

Toxin (Protein)


Gene name vagD Uniprot ID A0A3Q8D6L7
Locus tag HV092_RS26140 Protein ID WP_020804312.1
Coordinates 17086..17502 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag HV092_RS26135 Protein ID WP_001261276.1
Coordinates 16859..17089 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV092_RS26095 12399..12512 + 114 WP_014343462.1 hypothetical protein -
HV092_RS26100 12641..12898 + 258 WP_009310077.1 hypothetical protein -
HV092_RS26105 12956..13735 - 780 WP_023287113.1 site-specific integrase -
HV092_RS26110 13933..14949 - 1017 WP_009309921.1 hypothetical protein -
HV092_RS26115 14983..15318 - 336 WP_009309920.1 hypothetical protein -
HV092_RS26120 15368..15508 - 141 WP_162898808.1 hypothetical protein -
HV092_RS26125 15737..16042 - 306 WP_009309918.1 type II toxin-antitoxin system toxin CcdB -
HV092_RS26130 16044..16262 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA -
HV092_RS26135 16859..17089 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HV092_RS26140 17086..17502 + 417 WP_020804312.1 type II toxin-antitoxin system VapC family toxin Toxin
HV092_RS26145 17543..18421 - 879 WP_009309916.1 restriction endonuclease -
HV092_RS26150 19083..20360 + 1278 WP_009309915.1 HlyD family secretion protein -
HV092_RS26155 20350..22458 + 2109 WP_032427226.1 peptidase domain-containing ABC transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA 1..199458 199458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15154.59 Da        Isoelectric Point: 7.8637

>T165882 WP_020804312.1 NZ_CP058132:17086-17502 [Klebsiella quasipneumoniae]
MKKTWMLDTNICSFIMREQPESVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T165882 NZ_CP073994:1821139-1821393 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT165882 WP_001261276.1 NZ_CP058132:16859-17089 [Klebsiella quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT165882 NZ_CP073994:1820891-1821142 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3Q8D6L7

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References