Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 15737..16262 Replicon plasmid pRHBSTW-00138_2
Accession NZ_CP058132
Organism Klebsiella quasipneumoniae strain RHBSTW-00138

Toxin (Protein)


Gene name ccdB Uniprot ID A0A7H9GHC5
Locus tag HV092_RS26125 Protein ID WP_009309918.1
Coordinates 15737..16042 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag HV092_RS26130 Protein ID WP_006788213.1
Coordinates 16044..16262 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV092_RS26090 10811..11836 - 1026 WP_001101446.1 IS110 family transposase -
HV092_RS26095 12399..12512 + 114 WP_014343462.1 hypothetical protein -
HV092_RS26100 12641..12898 + 258 WP_009310077.1 hypothetical protein -
HV092_RS26105 12956..13735 - 780 WP_023287113.1 site-specific integrase -
HV092_RS26110 13933..14949 - 1017 WP_009309921.1 hypothetical protein -
HV092_RS26115 14983..15318 - 336 WP_009309920.1 hypothetical protein -
HV092_RS26120 15368..15508 - 141 WP_162898808.1 hypothetical protein -
HV092_RS26125 15737..16042 - 306 WP_009309918.1 type II toxin-antitoxin system toxin CcdB Toxin
HV092_RS26130 16044..16262 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HV092_RS26135 16859..17089 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
HV092_RS26140 17086..17502 + 417 WP_020804312.1 type II toxin-antitoxin system VapC family toxin -
HV092_RS26145 17543..18421 - 879 WP_009309916.1 restriction endonuclease -
HV092_RS26150 19083..20360 + 1278 WP_009309915.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA 1..199458 199458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11571.24 Da        Isoelectric Point: 6.4661

>T165881 WP_009309918.1 NZ_CP058132:c16042-15737 [Klebsiella quasipneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVVPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T165881 NZ_CP073994:1820891-1821142 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT165881 WP_006788213.1 NZ_CP058132:c16262-16044 [Klebsiella quasipneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT165881 NZ_CP073994:1821139-1821393 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H9GHC5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References