Detailed information of TA system TA165866

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 31659..32296
• Replicon: plasmid pRHBSTW-00140_2
• Accession: NZ_CP058129
• Organism: Enterobacter hormaechei strain RHBSTW-00140

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A9E6WXZ4
• Locus tag: HV094_RS23620
• Protein ID: WP_032662169.1
• Coordinates: 31659..32069 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A9E6WTF5
• Locus tag: HV094_RS23625
• Protein ID: WP_032662167.1
• Coordinates: 32066..32296 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV094_RS23600	27024..27374	+	351	WP_032662182.1	DUF305 domain-containing protein	-
HV094_RS23605	27560..28468	-	909	WP_032662179.1	HNH endonuclease	-
HV094_RS23610	29016..30038	-	1023	WP_040119796.1	helicase UvrD	-
HV094_RS23615	30023..31585	-	1563	WP_040119797.1	AAA family ATPase	-
HV094_RS23620	31659..32069	-	411	WP_032662169.1	PIN domain-containing protein	Toxin
HV094_RS23625	32066..32296	-	231	WP_032662167.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HV094_RS23630	32642..33775	+	1134	WP_063924579.1	hypothetical protein	-
HV094_RS23640	35149..35679	+	531	WP_013087127.1	hypothetical protein	-
HV094_RS23645	35698..36474	+	777	WP_015572054.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..87112	87112

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-127)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14729.03 Da
• Isoelectric Point: 5.7351

>T165866 WP_032662169.1 NZ_CP058129:c32069-31659 [Enterobacter hormaechei]
VNKMYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITWAEVSLASQEAGPAAQKLADAFAARLDAILPWDRTAV
DATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVR

Nucleotide

• Length: 411 bp

>T165866 NZ_CP073988:2600069-2600176 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8645.77 Da
• Isoelectric Point: 4.4692

>AT165866 WP_032662167.1 NZ_CP058129:c32296-32066 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMAERGDVVSDEGRVNL

Nucleotide

• Length: 231 bp

>AT165866 NZ_CP073988:c2600022-2599956 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT