Detailed information of TA system TA165864

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4119849..4120528
• Replicon: chromosome
• Accession: NZ_CP058128
• Organism: Enterobacter hormaechei strain RHBSTW-00140

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: R8WLP2
• Locus tag: HV094_RS19895
• Protein ID: WP_006812012.1
• Coordinates: 4119849..4120190 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: R8WMM2
• Locus tag: HV094_RS19900
• Protein ID: WP_006812013.1
• Coordinates: 4120211..4120528 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV094_RS19850	4115057..4115284	-	228	WP_032634595.1	TraR/DksA family transcriptional regulator	-
HV094_RS19855	4115284..4115517	-	234	WP_181236547.1	DUF2732 domain-containing protein	-
HV094_RS19860	4115585..4115926	-	342	WP_032610572.1	DUF5347 domain-containing protein	-
HV094_RS19865	4115890..4116090	-	201	WP_015386352.1	DUF2724 domain-containing protein	-
HV094_RS19870	4116098..4116607	-	510	WP_015386351.1	phage regulatory CII family protein	-
HV094_RS19875	4116642..4116878	-	237	WP_015386350.1	hypothetical protein	-
HV094_RS19880	4116980..4117594	+	615	WP_032610574.1	phage repressor protein CI	-
HV094_RS19885	4117606..4118505	+	900	WP_032610575.1	NAD-dependent DNA ligase	-
HV094_RS19890	4118515..4119528	+	1014	WP_032610576.1	tyrosine-type recombinase/integrase	-
HV094_RS19895	4119849..4120190	-	342	WP_006812012.1	TA system toxin CbtA family protein	Toxin
HV094_RS19900	4120211..4120528	-	318	WP_006812013.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV094_RS19905	4120548..4120769	-	222	WP_000691995.1	DUF987 domain-containing protein	-
HV094_RS19910	4120778..4121254	-	477	WP_047355118.1	RadC family protein	-
HV094_RS19915	4121270..4121728	-	459	WP_112020495.1	antirestriction protein	-
HV094_RS19920	4121831..4122070	-	240	WP_112020496.1	DUF905 domain-containing protein	-
HV094_RS19925	4122147..4122614	-	468	WP_063841896.1	hypothetical protein	-
HV094_RS19930	4122637..4123080	-	444	WP_001547764.1	phage transcriptional regulator	-
HV094_RS19935	4123080..4123316	-	237	WP_001115854.1	hypothetical protein	-
HV094_RS19940	4123353..4124054	-	702	WP_008324530.1	WYL domain-containing protein	-
HV094_RS19945	4124272..4125093	-	822	WP_097535357.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12989.07 Da
• Isoelectric Point: 10.4476

>T165864 WP_006812012.1 NZ_CP058128:c4120190-4119849 [Enterobacter hormaechei]
MKTLPATTQRAAKPCLAPVAVWQMLLTRLLKQHYGLTLNDTPFSEERVIQEHIDAGITLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRKNSVR

Nucleotide

• Length: 342 bp

>T165864 NZ_CP073988:2310242-2311564 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11874.45 Da
• Isoelectric Point: 7.0166

>AT165864 WP_006812013.1 NZ_CP058128:c4120528-4120211 [Enterobacter hormaechei]
MTHINWGLQRAITPRLGARLVQEGNRLHYLADRASITGRFSDTECRKLDETCPHFIRQMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQS

Nucleotide

• Length: 318 bp

>AT165864 NZ_CP073988:2309976-2310242 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: R8WLP2

Antitoxin

• AlphaFold DB: R8WMM2