Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4825..5561 Replicon plasmid pRHBSTW-00167_3
Accession NZ_CP058120
Organism Klebsiella michiganensis strain RHBSTW-00167

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag HV105_RS30220 Protein ID WP_003026803.1
Coordinates 5079..5561 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag HV105_RS30215 Protein ID WP_003026799.1
Coordinates 4825..5091 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV105_RS30190 1..1011 + 1011 WP_000200070.1 RepB family plasmid replication initiator protein -
HV105_RS30195 1709..2449 - 741 WP_001515717.1 tyrosine-type recombinase/integrase -
HV105_RS30200 2813..3781 - 969 WP_023307223.1 IS5-like element IS903B family transposase -
HV105_RS30205 3933..4136 + 204 WP_181250930.1 hemolysin expression modulator Hha -
HV105_RS30210 4150..4380 + 231 WP_023307222.1 hypothetical protein -
HV105_RS30215 4825..5091 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
HV105_RS30220 5079..5561 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
HV105_RS30225 5762..7165 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
HV105_RS30230 7194..7826 - 633 WP_001567369.1 hypothetical protein -
HV105_RS30235 8052..9398 + 1347 WP_077259381.1 ISNCY family transposase -
HV105_RS30240 9447..9842 + 396 WP_004143398.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..178580 178580
- inside IScluster/Tn - - 2813..11428 8615


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T165851 WP_003026803.1 NZ_CP058120:5079-5561 [Klebsiella michiganensis]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T165851 NZ_CP073984:c4288406-4288233 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT165851 WP_003026799.1 NZ_CP058120:4825-5091 [Klebsiella michiganensis]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT165851 NZ_CP073984:c4288730-4288434 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References