Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 146472..147115 Replicon plasmid pRHBSTW-00167_2
Accession NZ_CP058119
Organism Klebsiella michiganensis strain RHBSTW-00167

Toxin (Protein)


Gene name vagD Uniprot ID A0A5P6KDG5
Locus tag HV105_RS29720 Protein ID WP_021312768.1
Coordinates 146472..146888 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A5P6KGW8
Locus tag HV105_RS29725 Protein ID WP_021312767.1
Coordinates 146885..147115 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV105_RS29690 141773..142477 + 705 WP_001572363.1 IS6-like element IS26 family transposase -
HV105_RS29695 142732..143232 + 501 WP_021313689.1 non-heme ferritin-like protein -
HV105_RS29700 143380..143904 - 525 Protein_149 IS5-like element ISKpn26 family transposase -
HV105_RS29705 143940..145379 - 1440 Protein_150 IS66 family transposase -
HV105_RS29710 145444..146148 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
HV105_RS29715 146234..146374 + 141 WP_045286941.1 helix-turn-helix domain-containing protein -
HV105_RS29720 146472..146888 - 417 WP_021312768.1 type II toxin-antitoxin system VapC family toxin Toxin
HV105_RS29725 146885..147115 - 231 WP_021312767.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HV105_RS29730 147730..148029 + 300 WP_052433691.1 hypothetical protein -
HV105_RS29735 148084..148770 + 687 WP_052433690.1 hypothetical protein -
HV105_RS29740 148782..149564 + 783 WP_020277927.1 site-specific integrase -
HV105_RS29745 150099..150854 + 756 WP_001568031.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..223875 223875
- inside IScluster/Tn - - 111448..146148 34700


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15175.60 Da        Isoelectric Point: 7.2452

>T165850 WP_021312768.1 NZ_CP058119:c146888-146472 [Klebsiella michiganensis]
VNKIYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPDLVLEDWVR

Download         Length: 417 bp

>T165850 NZ_CP073984:c4186764-4186414 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8722.81 Da        Isoelectric Point: 4.4692

>AT165850 WP_021312767.1 NZ_CP058119:c147115-146885 [Klebsiella michiganensis]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT165850 NZ_CP073984:c4187009-4186758 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5P6KDG5

Antitoxin

Source ID Structure
AlphaFold DB A0A5P6KGW8

References