Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 120728..121464 Replicon plasmid pRHBSTW-00167_2
Accession NZ_CP058119
Organism Klebsiella michiganensis strain RHBSTW-00167

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag HV105_RS29540 Protein ID WP_003026803.1
Coordinates 120728..121210 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag HV105_RS29545 Protein ID WP_003026799.1
Coordinates 121198..121464 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV105_RS29525 117060..118582 + 1523 Protein_114 IS3 family transposase -
HV105_RS29530 118730..119125 - 396 WP_021312679.1 helix-turn-helix domain-containing protein -
HV105_RS29535 119174..120520 - 1347 WP_087794296.1 ISNCY family transposase -
HV105_RS29540 120728..121210 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
HV105_RS29545 121198..121464 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
HV105_RS29550 121640..121894 - 255 WP_004152108.1 hypothetical protein -
HV105_RS29555 121970..122227 - 258 WP_004152107.1 hypothetical protein -
HV105_RS29560 122276..122479 - 204 WP_004152106.1 hemolysin expression modulator Hha -
HV105_RS29565 122513..122881 - 369 WP_004152105.1 hypothetical protein -
HV105_RS29570 122925..123419 - 495 WP_004152104.1 DNA-binding protein -
HV105_RS29575 123652..124356 - 705 WP_023280904.1 IS6-like element IS26 family transposase -
HV105_RS29580 124421..125482 - 1062 Protein_125 DUF4158 domain-containing protein -
HV105_RS29585 125641..125931 + 291 WP_001247892.1 nucleotidyltransferase -
HV105_RS29590 125928..126329 + 402 WP_001293886.1 DUF86 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..223875 223875
- inside IScluster/Tn - - 111448..146148 34700


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T165849 WP_003026803.1 NZ_CP058119:c121210-120728 [Klebsiella michiganensis]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T165849 NZ_CP073984:3791006-3791284 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT165849 WP_003026799.1 NZ_CP058119:c121464-121198 [Klebsiella michiganensis]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT165849 NZ_CP073984:3790743-3791003 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References