Detailed information of TA system TA165848

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 78038..78573
• Replicon: plasmid pRHBSTW-00167_2
• Accession: NZ_CP058119
• Organism: Klebsiella michiganensis strain RHBSTW-00167

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A2N4Z1S3
• Locus tag: HV105_RS29285
• Protein ID: WP_020317119.1
• Coordinates: 78038..78325 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2N4Z1S6
• Locus tag: HV105_RS29290
• Protein ID: WP_020317117.1
• Coordinates: 78322..78573 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV105_RS29250	73265..73897	+	633	WP_017901447.1	ParA family protein	-
HV105_RS29255	73897..74271	+	375	WP_017901448.1	hypothetical protein	-
HV105_RS29260	74511..75485	+	975	WP_020317120.1	StbA protein	-
HV105_RS29265	75489..75881	+	393	WP_020317129.1	plasmid partitioning/stability family protein	-
HV105_RS29270	76295..77362	+	1068	WP_020317116.1	hypothetical protein	-
HV105_RS29275	77586..77861	-	276	WP_020317126.1	hypothetical protein	-
HV105_RS29280	77864..78034	-	171	WP_020317124.1	hypothetical protein	-
HV105_RS29285	78038..78325	-	288	WP_020317119.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV105_RS29290	78322..78573	-	252	WP_020317117.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HV105_RS29295	78737..80008	-	1272	WP_020317121.1	Y-family DNA polymerase	-
HV105_RS29300	80008..80433	-	426	WP_001776124.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
HV105_RS29305	80835..82322	+	1488	WP_161952885.1	group II intron reverse transcriptase/maturase	-
HV105_RS29310	82571..83542	+	972	WP_001568038.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..223875	223875

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11149.12 Da
• Isoelectric Point: 10.2246

>T165848 WP_020317119.1 NZ_CP058119:c78325-78038 [Klebsiella michiganensis]
MTYTVKFREDALKEWQKLDKSIQQQFAKKLKKCCDEPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDDQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T165848 NZ_CP073984:3567836-3568378 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9200.51 Da
• Isoelectric Point: 3.9507

>AT165848 WP_020317117.1 NZ_CP058119:c78573-78322 [Klebsiella michiganensis]
MPNIILSDTSASISELKKNPMATVSAGDGFPVAILNRNQPAFYCIPAELYEKMLDALDDQELAKLVLERSNQQLLDVDLD
TYL

Nucleotide

• Length: 252 bp

>AT165848 NZ_CP073984:3567541-3567852 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A2N4Z1S3

Antitoxin

• AlphaFold DB: A0A2N4Z1S6