Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 5270227..5270884 Replicon chromosome
Accession NZ_CP058118
Organism Klebsiella michiganensis strain RHBSTW-00167

Toxin (Protein)


Gene name cptA Uniprot ID J5U333
Locus tag HV105_RS25280 Protein ID WP_004854060.1
Coordinates 5270227..5270637 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID H3N295
Locus tag HV105_RS25285 Protein ID WP_004124953.1
Coordinates 5270618..5270884 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV105_RS25260 5266204..5267937 - 1734 WP_049075646.1 single-stranded-DNA-specific exonuclease RecJ -
HV105_RS25265 5267943..5268656 - 714 WP_009654991.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HV105_RS25270 5268679..5269575 - 897 WP_004105555.1 site-specific tyrosine recombinase XerD -
HV105_RS25275 5269697..5270218 + 522 WP_009654974.1 flavodoxin FldB -
HV105_RS25280 5270227..5270637 - 411 WP_004854060.1 protein YgfX Toxin
HV105_RS25285 5270618..5270884 - 267 WP_004124953.1 FAD assembly factor SdhE Antitoxin
HV105_RS25290 5271129..5272112 + 984 WP_004854063.1 tRNA-modifying protein YgfZ -
HV105_RS25295 5272288..5275086 + 2799 WP_181250239.1 transcriptional regulator DagR -
HV105_RS25300 5275207..5275632 + 426 WP_004854067.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16106.97 Da        Isoelectric Point: 10.9455

>T165847 WP_004854060.1 NZ_CP058118:c5270637-5270227 [Klebsiella michiganensis]
VVLWQSDLRISWRAQWFSLLMHGVVAALVLLMPWPLSYTPLWLILLSLVVFDCVRSQRRIHARQGEIKLLIDSRLRWQKA
EWDIIGTPWVINSGMLLRLRNTENQRTQHLWVAADSMDAGEWRDLRRLVLLKPTQE

Download         Length: 411 bp

>T165847 NZ_CP073984:3533863-3534081 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10411.88 Da        Isoelectric Point: 5.1296

>AT165847 WP_004124953.1 NZ_CP058118:c5270884-5270618 [Klebsiella michiganensis]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDGDKALFIRLLENDDPDLFNWLMNHGEPADAELQRMVKLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT165847 NZ_CP073984:3533463-3533837 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYA5

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3L9

References