Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 4163613..4164203 Replicon chromosome
Accession NZ_CP058118
Organism Klebsiella michiganensis strain RHBSTW-00167

Toxin (Protein)


Gene name pemK Uniprot ID -
Locus tag HV105_RS20125 Protein ID WP_049078225.1
Coordinates 4163613..4163945 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID J6I3K7
Locus tag HV105_RS20130 Protein ID WP_004852307.1
Coordinates 4163946..4164203 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV105_RS20100 4159026..4159283 + 258 WP_009653336.1 histidine kinase -
HV105_RS20105 4159415..4160869 + 1455 WP_004852297.1 AMP nucleosidase -
HV105_RS20115 4161833..4163290 - 1458 WP_032743138.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
HV105_RS20125 4163613..4163945 - 333 WP_049078225.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HV105_RS20130 4163946..4164203 - 258 WP_004852307.1 antitoxin Antitoxin
HV105_RS20135 4164543..4165457 - 915 WP_086531142.1 LysR family transcriptional regulator -
HV105_RS20140 4165558..4166556 + 999 WP_049078227.1 aldo/keto reductase -
HV105_RS20145 4166594..4167520 - 927 WP_049078228.1 LysR family transcriptional regulator -
HV105_RS20150 4167697..4168305 + 609 WP_181250029.1 glutathione S-transferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11837.72 Da        Isoelectric Point: 10.5834

>T165846 WP_049078225.1 NZ_CP058118:c4163945-4163613 [Klebsiella michiganensis]
MDRGEIWLVTLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLEGAGIKTTGVIRCDQPRTI
DIAARNGKRLERIPDAVVNDVLARLDAIFS

Download         Length: 333 bp

>T165846 NZ_CP073984:2753988-2754095 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9422.88 Da        Isoelectric Point: 4.4607

>AT165846 WP_004852307.1 NZ_CP058118:c4164203-4163946 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT165846 NZ_CP073984:c2753938-2753875 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTAAACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HDW8

References