Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1686203..1686822 Replicon chromosome
Accession NZ_CP058118
Organism Klebsiella michiganensis strain RHBSTW-00167

Toxin (Protein)


Gene name Hha Uniprot ID J5X5R4
Locus tag HV105_RS08040 Protein ID WP_004848333.1
Coordinates 1686203..1686421 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J4WQ74
Locus tag HV105_RS08045 Protein ID WP_009653563.1
Coordinates 1686448..1686822 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV105_RS08010 1682249..1682512 + 264 WP_004848323.1 type B 50S ribosomal protein L31 -
HV105_RS08015 1682512..1682652 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
HV105_RS08020 1682649..1683347 - 699 WP_128349260.1 GNAT family N-acetyltransferase -
HV105_RS08025 1683449..1684903 + 1455 WP_086530504.1 PLP-dependent aminotransferase family protein -
HV105_RS08030 1684878..1685348 - 471 WP_004848329.1 YlaC family protein -
HV105_RS08035 1685475..1686041 - 567 WP_181250569.1 maltose O-acetyltransferase -
HV105_RS08040 1686203..1686421 - 219 WP_004848333.1 hemolysin expression modulator Hha Toxin
HV105_RS08045 1686448..1686822 - 375 WP_009653563.1 Hha toxicity modulator TomB Antitoxin
HV105_RS08050 1687301..1690447 - 3147 WP_004848339.1 multidrug efflux RND transporter permease subunit -
HV105_RS08055 1690470..1691663 - 1194 WP_004136054.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8626.03 Da        Isoelectric Point: 8.9008

>T165836 WP_004848333.1 NZ_CP058118:c1686421-1686203 [Klebsiella michiganensis]
MSDKTLTKVDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T165836 NZ_CP073984:886144-886551 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTAATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14378.14 Da        Isoelectric Point: 4.8989

>AT165836 WP_009653563.1 NZ_CP058118:c1686822-1686448 [Klebsiella michiganensis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNASRENPVSLSC

Download         Length: 375 bp

>AT165836 NZ_CP073984:885897-886163 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4R4L0

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4R4I8

References