Detailed information of TA system TA165834

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 921153..921925
• Replicon: chromosome
• Accession: NZ_CP058118
• Organism: Klebsiella michiganensis strain RHBSTW-00167

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: HV105_RS04465
• Protein ID: WP_047724668.1
• Coordinates: 921536..921925 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HV105_RS04460
• Protein ID: WP_171972752.1
• Coordinates: 921153..921479 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV105_RS04425	916433..917065	-	633	WP_047724689.1	inovirus Gp2 family protein	-
HV105_RS04430	917703..918524	+	822	WP_047724687.1	hypothetical protein	-
HV105_RS04435	918768..919592	+	825	WP_047724685.1	DUF945 domain-containing protein	-
HV105_RS04440	919598..919846	+	249	WP_047725179.1	DUF905 domain-containing protein	-
HV105_RS04445	919926..920369	+	444	WP_047724684.1	antirestriction protein	-
HV105_RS04450	920381..920860	+	480	WP_047724681.1	DNA repair protein RadC	-
HV105_RS04455	920874..921095	+	222	WP_047724679.1	DUF987 domain-containing protein	-
HV105_RS04460	921153..921479	+	327	WP_171972752.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV105_RS04465	921536..921925	+	390	WP_047724668.1	TA system toxin CbtA family protein	Toxin
HV105_RS04470	922012..922857	+	846	WP_047724666.1	DUF4942 domain-containing protein	-
HV105_RS04475	923001..923798	+	798	WP_047724663.1	helix-turn-helix transcriptional regulator	-
HV105_RS04480	924150..925844	+	1695	WP_181250450.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	mrkA / mrkB / mrkC / mrkF / mrkJ	899567..948959	49392

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (3-111)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14425.51 Da
• Isoelectric Point: 8.0784

>T165834 WP_047724668.1 NZ_CP058118:921536-921925 [Klebsiella michiganensis]
MQTKLSPLMRAASSRPSPVDVWQTLLTCLLEHHYGLTLNDTPFSDEQVIQQHIEAGISLADALNFIVEKYELVRTDRPGF
SIMEQSPFITAIDILRARKATGLMNRGTYKEVTAITRGQHPQASTSGKR

Nucleotide

• Length: 390 bp

>T165834 NZ_CP073984:290094-290621 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12133.85 Da
• Isoelectric Point: 6.9459

>AT165834 WP_171972752.1 NZ_CP058118:921153-921479 [Klebsiella michiganensis]
ISEPWWGLRRSVSPCFGARLVQEGNRLHYLADRANFNGQFCDADLRHLDQAFPVLMKQLELMLTSGELNPRHQHCVTLYA
KGLTCEADTLGSHGYVYIAIYPTPVATA

Nucleotide

• Length: 327 bp

>AT165834 NZ_CP073984:289821-290087 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA