Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 46603..47339 Replicon plasmid pRHBSTW-00181_2
Accession NZ_CP058113
Organism Klebsiella grimontii strain RHBSTW-00181

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag HV112_RS27965 Protein ID WP_004098919.1
Coordinates 46857..47339 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A2J5Q9C0
Locus tag HV112_RS27960 Protein ID WP_004098921.1
Coordinates 46603..46869 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV112_RS27920 41860..42438 - 579 WP_009654302.1 hypothetical protein -
HV112_RS27925 42728..42991 + 264 WP_009310051.1 hypothetical protein -
HV112_RS27930 42988..43554 + 567 WP_009654312.1 hypothetical protein -
HV112_RS27935 43585..44079 + 495 WP_013023776.1 hypothetical protein -
HV112_RS27940 44123..44491 + 369 WP_063923539.1 hypothetical protein -
HV112_RS27945 44522..44725 + 204 WP_004098931.1 hemolysin expression modulator Hha -
HV112_RS27950 44774..45031 + 258 WP_004098928.1 hypothetical protein -
HV112_RS27955 45260..46228 - 969 WP_163467572.1 IS5 family transposase -
HV112_RS27960 46603..46869 + 267 WP_004098921.1 DUF1778 domain-containing protein Antitoxin
HV112_RS27965 46857..47339 + 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
HV112_RS27970 47540..48943 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
HV112_RS27975 48972..49604 - 633 WP_001567369.1 hypothetical protein -
HV112_RS27980 49831..51177 + 1347 WP_077255298.1 ISNCY family transposase -
HV112_RS27985 51236..52027 + 792 WP_003032641.1 tetratricopeptide repeat protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA 1..156264 156264
- inside IScluster/Tn - - 45260..58085 12825


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T165832 WP_004098919.1 NZ_CP058113:46857-47339 [Klebsiella grimontii]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T165832 NZ_CP073982:84381-85706 [Escherichia coli]
ATGAGCCGGAAACAGCAACGTCTGGTAATTTGGATGAACGGCATCAAAGTCGGGTACTGGGAAAAAAGCAAAGGGGTAGA
CAGCTTAGAATACCTTCCTGAATGGGTTGCTGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTGCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTGCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGATGAACCCACTGATTTATATTCCGTGAAGTATCACCCGCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGTATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGAGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGGCCAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAGACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCGTTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAGGTTCTGAGTAATTCAGACCGTGCTGAGC
GTGACAAAGCGCAGTTTTTTATGACGCAGATAATTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCAAACTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCCTGGCAGAAGTGCAAACTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACAGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGTGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCAGCAGCTTGCGGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTAGCTGAGAAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10105.49 Da        Isoelectric Point: 4.5141

>AT165832 WP_004098921.1 NZ_CP058113:46603-46869 [Klebsiella grimontii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEIACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT165832 NZ_CP073982:84083-84376 [Escherichia coli]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGCTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGCGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCCATCCTGGGCGTAAAAATCAGCTTCTCCTCGGCAACCACCTCTTCAGAGAGGAAG
CAGACGGAAGAAATGCTTAAATCAAACTCACCTGCACGACAGGAGGATTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A2J5Q9C0

References