Detailed information of TA system TA165831

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5728546..5729156
• Replicon: chromosome
• Accession: NZ_CP058112
• Organism: Klebsiella grimontii strain RHBSTW-00181

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A9E1GAR7
• Locus tag: HV112_RS27285
• Protein ID: WP_049086919.1
• Coordinates: 5728546..5728920 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HV112_RS27290
• Protein ID: WP_077253475.1
• Coordinates: 5728917..5729156 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV112_RS27270	5725701..5726603	+	903	WP_004122548.1	formate dehydrogenase subunit beta	-
HV112_RS27275	5726600..5727235	+	636	WP_004132860.1	formate dehydrogenase cytochrome b556 subunit	-
HV112_RS27280	5727579..5728508	+	930	WP_004132861.1	formate dehydrogenase accessory protein FdhE	-
HV112_RS27285	5728546..5728920	-	375	WP_049086919.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV112_RS27290	5728917..5729156	-	240	WP_077253475.1	ribbon-helix-helix domain-containing protein	Antitoxin
HV112_RS27295	5729362..5730279	+	918	WP_024360706.1	alpha/beta hydrolase	-
HV112_RS27300	5730297..5731238	-	942	WP_004127112.1	fatty acid biosynthesis protein FabY	-
HV112_RS27305	5731283..5731720	-	438	WP_004132870.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HV112_RS27310	5731717..5732577	-	861	WP_004127107.1	virulence factor BrkB family protein	-
HV112_RS27315	5732571..5733170	-	600	WP_024360709.1	glucose-1-phosphatase	-
HV112_RS27320	5733300..5734085	-	786	WP_064342034.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14111.45 Da
• Isoelectric Point: 8.4571

>T165831 WP_049086919.1 NZ_CP058112:c5728920-5728546 [Klebsiella grimontii]
MIKGPALFDTNILIDLFSGRVEAKQVLEAYPPQNAISLITWMEVMVGAKKYHQEHRTRIALSAFNIIDISQDIAERSVNL
RKEYGMKLPDAIILATAQIHRYELVTRNTKDFFGIPGVITPYHL

Nucleotide

• Length: 375 bp

>T165831 NZ_CP073981:c4477247-4476873 [Escherichia coli]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCTCATCTCCCGTAACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTAAATGACACACCGTTCTCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCACCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9293.49 Da
• Isoelectric Point: 4.3142

>AT165831 WP_077253475.1 NZ_CP058112:c5729156-5728917 [Klebsiella grimontii]
MMTGTNMGRILLDLSDDVIKRLEDLKQQRNLPRAELLREAVEQYLERQADTTISNALGIWQGCEEDGVEFERKLREEWQ

Nucleotide

• Length: 240 bp

>AT165831 NZ_CP073981:c4477705-4477337 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTCGGCGCTCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAAAGGGCTGACCTGCAAAGCCGACACCCTCGGTAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCAACATCCGAAACGAAAAAGTAA