Detailed information of TA system TA165830

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5134128..5134899
• Replicon: chromosome
• Accession: NZ_CP058112
• Organism: Klebsiella grimontii strain RHBSTW-00181

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2J4ZCW3
• Locus tag: HV112_RS24485
• Protein ID: WP_042944193.1
• Coordinates: 5134128..5134517 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HV112_RS24490
• Protein ID: WP_171840905.1
• Coordinates: 5134573..5134899 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV112_RS24455	5129182..5129976	+	795	WP_053064564.1	DNA/RNA non-specific endonuclease	-
HV112_RS24460	5130266..5130589	+	324	WP_064342117.1	endoribonuclease SymE	-
HV112_RS24465	5130736..5131170	+	435	WP_004098173.1	VOC family protein	-
HV112_RS24470	5131335..5132078	+	744	WP_064342116.1	hypothetical protein	-
HV112_RS24475	5132256..5133053	-	798	WP_042944196.1	helix-turn-helix transcriptional regulator	-
HV112_RS24480	5133196..5134041	-	846	WP_042944195.1	DUF4942 domain-containing protein	-
HV112_RS24485	5134128..5134517	-	390	WP_042944193.1	TA system toxin CbtA family protein	Toxin
HV112_RS24490	5134573..5134899	-	327	WP_171840905.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV112_RS24495	5134957..5135178	-	222	WP_004192297.1	DUF987 domain-containing protein	-
HV112_RS24500	5135192..5135671	-	480	WP_042944191.1	DNA repair protein RadC	-
HV112_RS24505	5135683..5136126	-	444	WP_042944189.1	antirestriction protein	-
HV112_RS24510	5136206..5136454	-	249	WP_049100007.1	DUF905 domain-containing protein	-
HV112_RS24515	5136460..5137284	-	825	WP_042944188.1	DUF945 domain-containing protein	-
HV112_RS24520	5137827..5138459	+	633	WP_049100008.1	inovirus Gp2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	5130736..5171132	40396

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (3-111)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14418.54 Da
• Isoelectric Point: 8.9846

>T165830 WP_042944193.1 NZ_CP058112:c5134517-5134128 [Klebsiella grimontii]
MQTKSPPFMRAASSRPSPVGVWQTLLTCLLEHHYGLTLSDTPFSDEQVIQQHIDAGISLVDALNFIVEKYELVRTDRPGF
SILTQSPFITPIDILRARKATGLMNRDTYKEVTAITRGQHPQVSAPGKR

Nucleotide

• Length: 390 bp

>T165830 NZ_CP073981:4276688-4276795 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12074.85 Da
• Isoelectric Point: 6.9367

>AT165830 WP_171840905.1 NZ_CP058112:c5134899-5134573 [Klebsiella grimontii]
ISEPWWGLRRSVSPCFGARLVQEGNRLHYLADRANVSGQFCDADLRHLDQAFPVLMKQLELMLTCGELNPRHQHCVTLYA
KGLTCEADTLGSHGYVYIAIYPTPVATA

Nucleotide

• Length: 327 bp

>AT165830 NZ_CP073981:c4276641-4276575 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A2J4ZCW3