Detailed information of TA system TA165818

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 940027..940684
• Replicon: chromosome
• Accession: NZ_CP058112
• Organism: Klebsiella grimontii strain RHBSTW-00181

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: A0A9E1DA00
• Locus tag: HV112_RS04570
• Protein ID: WP_024358614.1
• Coordinates: 940274..940684 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: H3N295
• Locus tag: HV112_RS04565
• Protein ID: WP_004124953.1
• Coordinates: 940027..940293 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV112_RS04550	935279..935704	-	426	WP_024358617.1	PTS sugar transporter subunit IIA	-
HV112_RS04555	935825..938623	-	2799	WP_064343189.1	transcriptional regulator DagR	-
HV112_RS04560	938799..939782	-	984	WP_024358615.1	tRNA-modifying protein YgfZ	-
HV112_RS04565	940027..940293	+	267	WP_004124953.1	FAD assembly factor SdhE	Antitoxin
HV112_RS04570	940274..940684	+	411	WP_024358614.1	protein YgfX	Toxin
HV112_RS04575	940693..941214	-	522	WP_024358613.1	flavodoxin FldB	-
HV112_RS04580	941336..942232	+	897	WP_004124942.1	site-specific tyrosine recombinase XerD	-
HV112_RS04585	942255..942968	+	714	WP_004134425.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
HV112_RS04590	942974..944707	+	1734	WP_049090244.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16135.97 Da
• Isoelectric Point: 10.9455

>T165818 WP_024358614.1 NZ_CP058112:940274-940684 [Klebsiella grimontii]
VVLWQSDLRISWRAQWFSLLMHGVVAALVLLMPWPLSYTPLWLILLSLVVFDCVRSQRRIHARQGEIKLLIDSRLRWQKA
EWEIIGTPWVINSGMLLRLRNTENQRTQHLWVAADSMDAGEWRDLRRLVLQKPTQE

Nucleotide

• Length: 411 bp

>T165818 NZ_CP073981:2279542-2279856 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10411.88 Da
• Isoelectric Point: 5.1296

>AT165818 WP_004124953.1 NZ_CP058112:940027-940293 [Klebsiella grimontii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDGDKALFIRLLENDDPDLFNWLMNHGEPADAELQRMVKLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT165818 NZ_CP073981:2279853-2280269 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAATCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3H3L9