Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3559561..3560215 Replicon chromosome
Accession NZ_CP058057
Organism Escherichia fergusonii strain RHB02-C22

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag HVV54_RS17175 Protein ID WP_000244781.1
Coordinates 3559561..3559968 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HVV54_RS17180 Protein ID WP_000354046.1
Coordinates 3559949..3560215 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVV54_RS17155 3555518..3557251 - 1734 WP_000813234.1 single-stranded-DNA-specific exonuclease RecJ -
HVV54_RS17160 3557257..3557967 - 711 WP_104917537.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HVV54_RS17165 3557992..3558888 - 897 WP_000806645.1 site-specific tyrosine recombinase XerD -
HVV54_RS17170 3559000..3559521 + 522 WP_001055874.1 flavodoxin FldB -
HVV54_RS17175 3559561..3559968 - 408 WP_000244781.1 protein YgfX Toxin
HVV54_RS17180 3559949..3560215 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HVV54_RS17185 3560467..3561447 + 981 WP_181202810.1 tRNA-modifying protein YgfZ -
HVV54_RS17190 3561524..3562183 - 660 WP_000250283.1 hemolysin III family protein -
HVV54_RS17195 3562347..3562658 - 312 WP_001182952.1 N(4)-acetylcytidine aminohydrolase -
HVV54_RS17200 3562703..3564136 + 1434 WP_148047390.1 6-phospho-beta-glucosidase BglA -
HVV54_RS17205 3564184..3565077 - 894 WP_000819362.1 transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T165805 WP_000244781.1 NZ_CP058057:c3559968-3559561 [Escherichia fergusonii]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T165805 NZ_CP073974:c3566559-3566095 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT165805 WP_000354046.1 NZ_CP058057:c3560215-3559949 [Escherichia fergusonii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT165805 NZ_CP073974:c3566894-3566559 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References