Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 2027..2562 Replicon plasmid pRHB10-C04_2
Accession NZ_CP057919
Organism Escherichia fergusonii strain RHB10-C04

Toxin (Protein)


Gene name relE Uniprot ID T2HS21
Locus tag HVW38_RS22015 Protein ID WP_000222775.1
Coordinates 2275..2562 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID T2HSA2
Locus tag HVW38_RS22010 Protein ID WP_001139207.1
Coordinates 2027..2278 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVW38_RS22005 46..1077 + 1032 WP_000907867.1 replication initiation protein -
HVW38_RS22010 2027..2278 + 252 WP_001139207.1 plasmid stabilization protein Antitoxin
HVW38_RS22015 2275..2562 + 288 WP_000222775.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HVW38_RS22020 2572..3117 + 546 WP_181576141.1 hypothetical protein -
HVW38_RS22025 3769..3882 - 114 Protein_4 proQ/FINO family protein -
HVW38_RS22030 3921..5459 - 1539 WP_000998048.1 IS66-like element ISEc8 family transposase -
HVW38_RS22035 5509..5856 - 348 WP_000612591.1 IS66 family insertion sequence element accessory protein TnpB -
HVW38_RS22040 5853..6233 - 381 WP_001171554.1 IS66 family insertion sequence hypothetical protein -
HVW38_RS22045 6321..6506 - 186 Protein_8 proQ/FINO family protein -
HVW38_RS22050 6601..7347 - 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..114980 114980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11142.17 Da        Isoelectric Point: 10.6261

>T165780 WP_000222775.1 NZ_CP057919:2275-2562 [Escherichia fergusonii]
MTYTVKFRDDALREWLKLDKSVQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSHVYNLASERMR

Download         Length: 288 bp

>T165780 NZ_CP057919:2275-2562 [Escherichia fergusonii]
ATGACTTATACGGTTAAATTCAGGGATGATGCCCTCAGGGAATGGCTGAAACTGGATAAATCCGTTCAACAACAGTTCGC
AAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCTTCAGCAAAACTACGTGGATTAAAAGATTGCTACAAAATAA
AACTTCGCGCATCAGGTTTTCGTCTGGTCTATCAGGTGATTGATGACATGTTAATTATTGCGGTTGTCGCAGTAGGTAAA
CGTGAACGTAGTCATGTTTACAATCTCGCCAGCGAGAGAATGAGATAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9216.45 Da        Isoelectric Point: 4.0927

>AT165780 WP_001139207.1 NZ_CP057919:2027-2278 [Escherichia fergusonii]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDAMDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp

>AT165780 NZ_CP057919:2027-2278 [Escherichia fergusonii]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAACTGAAAAAAAATCCAATGGCGACAGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCTGCATTCTACTGTGTTCCTGCTGAATTGTATGAAAGGATGC
TTGATGCTATGGATGACCAGGAGCTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTACATGATGTGGACCTGGAT
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB T2HS21

Antitoxin

Source ID Structure
AlphaFold DB A0A754EQ65

References