Detailed information of TA system TA165775

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3128143..3128762
• Replicon: chromosome
• Accession: NZ_CP057918
• Organism: Escherichia fergusonii strain RHB10-C04

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: V1H8E6
• Locus tag: HVW38_RS14980
• Protein ID: WP_001280991.1
• Coordinates: 3128544..3128762 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: HVW38_RS14975
• Protein ID: WP_104917851.1
• Coordinates: 3128143..3128517 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVW38_RS14965	3123275..3124468	+	1194	WP_002431407.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
HVW38_RS14970	3124491..3127640	+	3150	WP_001132493.1	multidrug efflux RND transporter permease subunit	-
HVW38_RS14975	3128143..3128517	+	375	WP_104917851.1	Hha toxicity modulator TomB	Antitoxin
HVW38_RS14980	3128544..3128762	+	219	WP_001280991.1	hemolysin expression modulator Hha	Toxin
HVW38_RS14985	3129259..3129729	+	471	WP_002431406.1	YlaC family protein	-
HVW38_RS14990	3129868..3131418	+	1551	WP_001260376.1	EAL domain-containing protein	-
HVW38_RS14995	3131460..3131813	-	354	WP_000878140.1	DUF1428 domain-containing protein	-
HVW38_RS15005	3132192..3132503	+	312	WP_000409911.1	MGMT family protein	-
HVW38_RS15010	3132534..3133106	-	573	WP_000779831.1	YbaY family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8613.97 Da
• Isoelectric Point: 8.9008

>T165775 WP_001280991.1 NZ_CP057918:3128544-3128762 [Escherichia fergusonii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T165775 NZ_CP073962:c1905918-1905541 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14511.31 Da
• Isoelectric Point: 4.8989

>AT165775 WP_104917851.1 NZ_CP057918:3128143-3128517 [Escherichia fergusonii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT165775 NZ_CP073962:c1906376-1906008 [Escherichia coli]
GTGTCAGACACACTTTCCGGGGCAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• PDB: 4ICG