Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4707635..4708251 Replicon chromosome
Accession NZ_CP057864
Organism Citrobacter freundii strain RHB12-C19

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag HVW68_RS22315 Protein ID WP_003028682.1
Coordinates 4707635..4708009 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag HVW68_RS22320 Protein ID WP_043018956.1
Coordinates 4708009..4708251 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVW68_RS22300 4705138..4706040 + 903 WP_003028691.1 formate dehydrogenase O subunit beta -
HVW68_RS22305 4706037..4706672 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
HVW68_RS22310 4706669..4707598 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
HVW68_RS22315 4707635..4708009 - 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
HVW68_RS22320 4708009..4708251 - 243 WP_043018956.1 ribbon-helix-helix domain-containing protein Antitoxin
HVW68_RS22325 4708457..4709365 + 909 WP_181825860.1 alpha/beta hydrolase -
HVW68_RS22330 4709516..4710457 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
HVW68_RS22335 4710502..4710939 - 438 WP_003028671.1 D-tyrosyl-tRNA(Tyr) deacylase -
HVW68_RS22340 4710936..4711808 - 873 WP_003028669.1 virulence factor BrkB family protein -
HVW68_RS22345 4711802..4712401 - 600 WP_016151258.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T165739 WP_003028682.1 NZ_CP057864:c4708009-4707635 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

>T165739 NZ_CP073955:c8386-8006 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT165739 WP_043018956.1 NZ_CP057864:c4708251-4708009 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT165739 NZ_CP073955:c8607-8386 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References