Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3577233..3577853 Replicon chromosome
Accession NZ_CP057864
Organism Citrobacter freundii strain RHB12-C19

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HVW68_RS17095 Protein ID WP_002892050.1
Coordinates 3577635..3577853 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag HVW68_RS17090 Protein ID WP_003021733.1
Coordinates 3577233..3577607 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVW68_RS17080 3572379..3573572 + 1194 WP_003835922.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HVW68_RS17085 3573595..3576744 + 3150 WP_003021736.1 multidrug efflux RND transporter permease subunit -
HVW68_RS17090 3577233..3577607 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
HVW68_RS17095 3577635..3577853 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HVW68_RS17100 3578035..3578586 + 552 WP_032936967.1 maltose O-acetyltransferase -
HVW68_RS17105 3578703..3579173 + 471 WP_003021724.1 YlaC family protein -
HVW68_RS17110 3579252..3579392 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
HVW68_RS17115 3579394..3579654 - 261 WP_003021719.1 type B 50S ribosomal protein L31 -
HVW68_RS17120 3579843..3581396 + 1554 WP_003835927.1 EAL domain-containing protein -
HVW68_RS17125 3581448..3581801 - 354 WP_181825700.1 DUF1428 family protein -
HVW68_RS17130 3581866..3582495 - 630 WP_181825701.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T165736 WP_002892050.1 NZ_CP057864:3577635-3577853 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T165736 NZ_CP073953:c4629743-4629432 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT165736 WP_003021733.1 NZ_CP057864:3577233-3577607 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT165736 NZ_CP073953:c4629431-4629141 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References