Detailed information of TA system TA165735

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 2872333..2872923
• Replicon: chromosome
• Accession: NZ_CP057864
• Organism: Citrobacter freundii strain RHB12-C19

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: HVW68_RS13785
• Protein ID: WP_181825620.1
• Coordinates: 2872333..2872665 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A0J1NBD4
• Locus tag: HVW68_RS13790
• Protein ID: WP_003836694.1
• Coordinates: 2872666..2872923 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVW68_RS13760	2868025..2869512	-	1488	WP_003846749.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
HVW68_RS13770	2869889..2870839	-	951	WP_003846751.1	HTH-type transcriptional regulator Cbl	-
HVW68_RS13775	2870941..2871858	-	918	WP_003030855.1	nitrogen assimilation transcriptional regulator NAC	-
HVW68_RS13785	2872333..2872665	-	333	WP_181825620.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HVW68_RS13790	2872666..2872923	-	258	WP_003836694.1	hypothetical protein	Antitoxin
HVW68_RS13795	2873538..2877437	+	3900	WP_181825621.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2861936..2893113	31177

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11791.54 Da
• Isoelectric Point: 7.8365

>T165735 WP_181825620.1 NZ_CP057864:c2872665-2872333 [Citrobacter freundii]
MDRGEIWLVSLDPTAGHEQSGKRPVLIVSPASFNKFTRLPVVVPVTSGGNFARTAGFTVSLDDAGTKTTGVIRCDQPRTI
DMGARNGKCLERIPEAVVNEVLARLEAILS

Nucleotide

• Length: 333 bp

>T165735 NZ_CP073953:c4352708-4352535 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9428.89 Da
• Isoelectric Point: 4.3440

>AT165735 WP_003836694.1 NZ_CP057864:c2872923-2872666 [Citrobacter freundii]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSDEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT165735 NZ_CP073953:c4353032-4352736 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0J1NBD4