Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 826867..827521 Replicon chromosome
Accession NZ_CP057864
Organism Citrobacter freundii strain RHB12-C19

Toxin (Protein)


Gene name cptA Uniprot ID A0A0J1NHY7
Locus tag HVW68_RS04040 Protein ID WP_003026936.1
Coordinates 827114..827521 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A6H3AT26
Locus tag HVW68_RS04035 Protein ID WP_003026938.1
Coordinates 826867..827133 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVW68_RS04010 822068..823501 - 1434 WP_003838263.1 6-phospho-beta-glucosidase BglA -
HVW68_RS04015 823622..824350 - 729 WP_003838265.1 MurR/RpiR family transcriptional regulator -
HVW68_RS04020 824403..824714 + 312 WP_003026949.1 N(4)-acetylcytidine aminohydrolase -
HVW68_RS04025 824877..825536 + 660 WP_003838267.1 hemolysin III family protein -
HVW68_RS04030 825630..826610 - 981 WP_003026944.1 tRNA-modifying protein YgfZ -
HVW68_RS04035 826867..827133 + 267 WP_003026938.1 FAD assembly factor SdhE Antitoxin
HVW68_RS04040 827114..827521 + 408 WP_003026936.1 protein YgfX Toxin
HVW68_RS04045 827566..828087 - 522 WP_003026933.1 flavodoxin FldB -
HVW68_RS04050 828201..829097 + 897 WP_003026928.1 site-specific tyrosine recombinase XerD -
HVW68_RS04055 829121..829834 + 714 WP_003825520.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HVW68_RS04060 829840..831573 + 1734 WP_016150943.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15953.89 Da        Isoelectric Point: 11.4054

>T165724 WP_003026936.1 NZ_CP057864:827114-827521 [Citrobacter freundii]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWSIVSAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLMLQKAKQK

Download         Length: 408 bp

>T165724 NZ_CP073953:1791133-1791384 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATAGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10558.09 Da        Isoelectric Point: 5.1700

>AT165724 WP_003026938.1 NZ_CP057864:826867-827133 [Citrobacter freundii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAELEQMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT165724 NZ_CP073953:1791381-1791635 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1NHY7

Antitoxin

Source ID Structure
AlphaFold DB A0A6H3AT26

References