Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 52612..53255 Replicon plasmid pRHB16-C24_2
Accession NZ_CP057723
Organism Klebsiella pneumoniae strain RHB16-C24

Toxin (Protein)


Gene name vagD Uniprot ID A0A6H2GRP8
Locus tag HVX23_RS25030 Protein ID WP_023292131.1
Coordinates 52839..53255 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A6B7Q5S4
Locus tag HVX23_RS25025 Protein ID WP_021312476.1
Coordinates 52612..52842 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVX23_RS25000 47679..48856 + 1178 Protein_41 IS3 family transposase -
HVX23_RS25005 49238..50023 - 786 WP_050485696.1 site-specific integrase -
HVX23_RS25010 50069..50590 - 522 WP_094313782.1 hypothetical protein -
HVX23_RS25015 50648..51040 - 393 WP_170933338.1 hypothetical protein -
HVX23_RS25020 51077..52021 - 945 WP_094313783.1 hypothetical protein -
HVX23_RS25025 52612..52842 + 231 WP_021312476.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HVX23_RS25030 52839..53255 + 417 WP_023292131.1 type II toxin-antitoxin system VapC family toxin Toxin
HVX23_RS25035 53290..53535 + 246 WP_065801591.1 hypothetical protein -
HVX23_RS25040 53599..53853 + 255 WP_095153353.1 hypothetical protein -
HVX23_RS25045 54410..55416 + 1007 Protein_50 IS110 family transposase -
HVX23_RS25050 55695..55943 + 249 WP_065801593.1 hypothetical protein -
HVX23_RS25055 55948..56520 + 573 WP_094309628.1 hypothetical protein -
HVX23_RS25060 56551..57045 + 495 WP_094313750.1 DNA-binding protein -
HVX23_RS25065 57106..57309 + 204 WP_004150739.1 hemolysin expression modulator Hha -
HVX23_RS25070 57358..57567 + 210 WP_094308932.1 hypothetical protein -
HVX23_RS25075 57597..57905 - 309 Protein_56 DDE-type integrase/transposase/recombinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - iutA / iucD / iucC / iucB / iucA / cseA 1..147522 147522


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14975.40 Da        Isoelectric Point: 8.5464

>T165719 WP_023292131.1 NZ_CP057723:52839-53255 [Klebsiella pneumoniae]
VKKTFMLDTNICSFIMREQPAAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDATTEIKVALRQAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVN

Download         Length: 417 bp

>T165719 NZ_CP073953:291626-292153 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8723.76 Da        Isoelectric Point: 4.3410

>AT165719 WP_021312476.1 NZ_CP057723:52612-52842 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDL

Download         Length: 231 bp

>AT165719 NZ_CP073953:291353-291619 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6H2GRP8

Antitoxin

Source ID Structure
AlphaFold DB A0A6B7Q5S4

References