Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5056827..5057452 Replicon chromosome
Accession NZ_CP057722
Organism Klebsiella pneumoniae strain RHB16-C24

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag HVX23_RS24430 Protein ID WP_004187928.1
Coordinates 5056827..5057210 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HVX23_RS24435 Protein ID WP_004150355.1
Coordinates 5057210..5057452 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVX23_RS24415 5054193..5055095 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HVX23_RS24420 5055092..5055727 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HVX23_RS24425 5055724..5056653 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HVX23_RS24430 5056827..5057210 - 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
HVX23_RS24435 5057210..5057452 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HVX23_RS24440 5057646..5058563 + 918 WP_004187929.1 alpha/beta hydrolase -
HVX23_RS24445 5058577..5059518 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HVX23_RS24450 5059563..5060000 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HVX23_RS24455 5059997..5060857 - 861 WP_004146232.1 virulence factor BrkB family protein -
HVX23_RS24460 5060851..5061450 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T165718 WP_004187928.1 NZ_CP057722:c5057210-5056827 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

>T165718 NZ_CP073953:270063-270293 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT165718 WP_004150355.1 NZ_CP057722:c5057452-5057210 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT165718 NZ_CP073953:270290-270649 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References