Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4483008..4483818 Replicon chromosome
Accession NZ_CP057722
Organism Klebsiella pneumoniae strain RHB16-C24

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag HVX23_RS21765 Protein ID WP_004178461.1
Coordinates 4483008..4483541 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag HVX23_RS21770 Protein ID WP_002887278.1
Coordinates 4483552..4483818 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVX23_RS21760 4481839..4482960 + 1122 WP_004186850.1 cupin domain-containing protein -
HVX23_RS21765 4483008..4483541 - 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
HVX23_RS21770 4483552..4483818 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
HVX23_RS21775 4483921..4485354 - 1434 WP_116954916.1 Cu(+)/Ag(+) sensor histidine kinase -
HVX23_RS21780 4485344..4486027 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
HVX23_RS21785 4486199..4487584 + 1386 WP_117043268.1 efflux transporter outer membrane subunit -
HVX23_RS21790 4487602..4487946 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T165715 WP_004178461.1 NZ_CP057722:c4483541-4483008 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T165715 NZ_CP073953:194430-194537 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGTATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT165715 WP_002887278.1 NZ_CP057722:c4483818-4483552 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT165715 NZ_CP073953:c194372-194316 [Escherichia coli]
CAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References