Detailed information of TA system TA165712

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2527127..2528715
• Replicon: chromosome
• Accession: NZ_CP057722
• Organism: Klebsiella pneumoniae strain RHB16-C24

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A486JB51
• Locus tag: HVX23_RS12295
• Protein ID: WP_023316847.1
• Coordinates: 2527127..2528449 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HVX23_RS12300
• Protein ID: WP_004176198.1
• Coordinates: 2528449..2528715 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVX23_RS12280	2522183..2524684	+	2502	WP_048333660.1	fimbrial biogenesis outer membrane usher protein	-
HVX23_RS12285	2524672..2525676	+	1005	WP_032421320.1	type 1 fimbrial protein	-
HVX23_RS12290	2525818..2527110	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
HVX23_RS12295	2527127..2528449	-	1323	WP_023316847.1	type II toxin-antitoxin system HipA family toxin	Toxin
HVX23_RS12300	2528449..2528715	-	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HVX23_RS12305	2528981..2529307	-	327	WP_019705664.1	hydroxyisourate hydrolase	-
HVX23_RS12310	2529304..2529804	-	501	WP_019705663.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
HVX23_RS12315	2529782..2531161	-	1380	WP_100111711.1	purine permease	-
HVX23_RS12320	2531507..2532661	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
HVX23_RS12325	2532642..2533565	-	924	WP_040224150.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49141.44 Da
• Isoelectric Point: 7.5357

>T165712 WP_023316847.1 NZ_CP057722:c2528449-2527127 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T165712 NZ_CP073949:c4089517-4089227 [Escherichia coli]
ATGAACTATGAACTGGCTTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGGTGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTACTGGATTTGCCGGACTGCTACAAAATTA
AGCTTCGCACACTGGGATATCGCCGGGTCTATCAGGTCAATGATAAAGAGTTACTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGTGACAAAACGGCTGGATGAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT165712 WP_004176198.1 NZ_CP057722:c2528715-2528449 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT165712 NZ_CP073949:c4089749-4089507 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAGGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Structures

Toxin

• AlphaFold DB: A0A486JB51