Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3591763..3592313 Replicon chromosome
Accession NZ_CP057692
Organism Escherichia fergusonii strain RHB17-C11

Toxin (Protein)


Gene name ccdB Uniprot ID F0JPU1
Locus tag HVX29_RS17395 Protein ID WP_001160963.1
Coordinates 3591999..3592313 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag HVX29_RS17390 Protein ID WP_000125566.1
Coordinates 3591763..3591996 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVX29_RS17365 3586835..3587122 + 288 WP_000203741.1 ferredoxin-like protein FixX -
HVX29_RS17370 3587181..3588512 + 1332 WP_181812687.1 MFS transporter -
HVX29_RS17375 3588620..3589150 + 531 WP_000600745.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
HVX29_RS17380 3589143..3591005 + 1863 WP_000377099.1 glutathione-regulated potassium-efflux system protein KefC -
HVX29_RS17385 3591198..3591677 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
HVX29_RS17390 3591763..3591996 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HVX29_RS17395 3591999..3592313 + 315 WP_001160963.1 CcdB family protein Toxin
HVX29_RS17400 3592310..3593158 - 849 WP_000257179.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
HVX29_RS17405 3593165..3593542 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
HVX29_RS17410 3593545..3594366 - 822 WP_001065390.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
HVX29_RS17415 3594363..3595352 - 990 WP_000241251.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
HVX29_RS17420 3595352..3596638 - 1287 WP_000800490.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11722.70 Da        Isoelectric Point: 8.0666

>T165696 WP_001160963.1 NZ_CP057692:3591999-3592313 [Escherichia fergusonii]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSHIRPPERLNPILLLVDGKEYVLMTHETATVPVNTLG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T165696 NZ_CP073949:639551-639865 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT165696 WP_000125566.1 NZ_CP057692:3591763-3591996 [Escherichia fergusonii]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT165696 NZ_CP073949:639862-640278 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB F0JPU1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References