Detailed information of TA system TA165669

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 42612..43137
• Replicon: plasmid pRHB18-C04_2
• Accession: NZ_CP057682
• Organism: Escherichia fergusonii strain RHB18-C04

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: HVX35_RS22330
• Protein ID: WP_175283076.1
• Coordinates: 42832..43137 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: HVX35_RS22325
• Protein ID: WP_181464566.1
• Coordinates: 42612..42830 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVX35_RS22320	38932..42063	+	3132	WP_181464565.1	hypothetical protein	-
HVX35_RS22325	42612..42830	+	219	WP_181464566.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
HVX35_RS22330	42832..43137	+	306	WP_175283076.1	type II toxin-antitoxin system toxin CcdB	Toxin
HVX35_RS22335	43138..43947	+	810	WP_181464567.1	site-specific integrase	-
HVX35_RS22340	44084..44359	-	276	WP_000239529.1	hypothetical protein	-
HVX35_RS22345	44353..44997	-	645	WP_000633913.1	AAA family ATPase	-
HVX35_RS22350	45226..46197	+	972	WP_181464568.1	plasmid segregation protein parM	-
HVX35_RS22355	46202..46594	+	393	WP_181464569.1	plasmid partitioning/stability family protein	-
HVX35_RS22360	46599..47870	-	1272	Protein_43	translesion error-prone DNA polymerase V subunit UmuC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..117379	117379

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11682.45 Da
• Isoelectric Point: 6.4674

>T165669 WP_175283076.1 NZ_CP057682:42832-43137 [Escherichia fergusonii]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVSVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T165669 NZ_CP073943:2587555-2587662 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8353.28 Da
• Isoelectric Point: 4.7063

>AT165669 WP_181464566.1 NZ_CP057682:42612-42830 [Escherichia fergusonii]
MKQRITVTVDSNSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT165669 NZ_CP073943:c2587508-2587442 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT