Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3521151..3521701 Replicon chromosome
Accession NZ_CP057681
Organism Escherichia fergusonii strain RHB18-C04

Toxin (Protein)


Gene name ccdB Uniprot ID F0JPU1
Locus tag HVX35_RS17150 Protein ID WP_001160963.1
Coordinates 3521387..3521701 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag HVX35_RS17145 Protein ID WP_000125566.1
Coordinates 3521151..3521384 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVX35_RS17120 3516223..3516510 + 288 WP_000203741.1 ferredoxin-like protein FixX -
HVX35_RS17125 3516569..3517900 + 1332 WP_181198739.1 MFS transporter -
HVX35_RS17130 3518008..3518538 + 531 WP_000600701.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
HVX35_RS17135 3518531..3520393 + 1863 WP_104919956.1 glutathione-regulated potassium-efflux system protein KefC -
HVX35_RS17140 3520586..3521065 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
HVX35_RS17145 3521151..3521384 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HVX35_RS17150 3521387..3521701 + 315 WP_001160963.1 CcdB family protein Toxin
HVX35_RS17155 3521698..3522546 - 849 WP_000257179.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
HVX35_RS17160 3522553..3522930 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
HVX35_RS17165 3522933..3523754 - 822 WP_001065390.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
HVX35_RS17170 3523751..3524740 - 990 WP_046076786.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
HVX35_RS17175 3524740..3526026 - 1287 WP_181198740.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11722.70 Da        Isoelectric Point: 8.0666

>T165664 WP_001160963.1 NZ_CP057681:3521387-3521701 [Escherichia fergusonii]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSHIRPPERLNPILLLVDGKEYVLMTHETATVPVNTLG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T165664 NZ_CP073943:1944090-1944193 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT165664 WP_000125566.1 NZ_CP057681:3521151-3521384 [Escherichia fergusonii]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT165664 NZ_CP073943:c1944231-1943960 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB F0JPU1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References