Detailed information of TA system TA165661

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 3331176..3331698
• Replicon: chromosome
• Accession: NZ_CP057681
• Organism: Escherichia fergusonii strain RHB18-C04

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HVX35_RS16125
• Protein ID: WP_181198684.1
• Coordinates: 3331408..3331698 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0SC69
• Locus tag: HVX35_RS16120
• Protein ID: WP_000212715.1
• Coordinates: 3331176..3331418 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVX35_RS16100	3327093..3328466	+	1374	WP_001219809.1	UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase	-
HVX35_RS16105	3328549..3329100	-	552	WP_000166270.1	ribosome-associated protein	-
HVX35_RS16110	3329194..3330546	+	1353	WP_001162164.1	metalloprotease PmbA	-
HVX35_RS16115	3330599..3330985	+	387	WP_001232246.1	cytochrome b562	-
HVX35_RS16120	3331176..3331418	+	243	WP_000212715.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HVX35_RS16125	3331408..3331698	+	291	WP_181198684.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HVX35_RS16130	3331699..3332163	-	465	WP_001106232.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
HVX35_RS16135	3332321..3334459	-	2139	WP_046076417.1	anaerobic ribonucleoside-triphosphate reductase	-
HVX35_RS16140	3334853..3336508	-	1656	WP_046076416.1	alpha,alpha-phosphotrehalase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-72)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11324.16 Da
• Isoelectric Point: 10.0464

>T165661 WP_181198684.1 NZ_CP057681:3331408-3331698 [Escherichia fergusonii]
MNYELAFDPRALKEWQKLGVTVREQFKKKLGDVLKRPRNPSAKLRDLPDCYEIKLRTLGYRRVYQVNDKELLVLVIAIGK
RENSAVYEDATKRLDE

Nucleotide

• Length: 291 bp

>T165661 NZ_CP073943:1285607-1286005 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9125.56 Da
• Isoelectric Point: 5.1369

>AT165661 WP_000212715.1 NZ_CP057681:3331176-3331418 [Escherichia fergusonii]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAENGRMPVKTLTVSDSEEALLRTVRERLANPQEAIKVRLDEL

Nucleotide

• Length: 243 bp

>AT165661 NZ_CP073943:1285380-1285607 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9H4B3