Detailed information of TA system TA165634

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3592457..3593007
• Replicon: chromosome
• Accession: NZ_CP057657
• Organism: Escherichia fergusonii strain RHB19-C05

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: HVX45_RS17485
• Protein ID: WP_032183189.1
• Coordinates: 3592693..3593007 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: HVX45_RS17480
• Protein ID: WP_000125566.1
• Coordinates: 3592457..3592690 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVX45_RS17455	3587529..3587816	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
HVX45_RS17460	3587876..3589207	+	1332	WP_001183184.1	MFS transporter	-
HVX45_RS17465	3589314..3589844	+	531	WP_032183187.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
HVX45_RS17470	3589837..3591699	+	1863	WP_000377102.1	glutathione-regulated potassium-efflux system protein KefC	-
HVX45_RS17475	3591892..3592371	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
HVX45_RS17480	3592457..3592690	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
HVX45_RS17485	3592693..3593007	+	315	WP_032183189.1	CcdB family protein	Toxin
HVX45_RS17490	3593004..3593852	-	849	WP_000257189.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
HVX45_RS17495	3593859..3594236	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
HVX45_RS17500	3594239..3595060	-	822	WP_001065375.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
HVX45_RS17505	3595057..3596046	-	990	WP_046083039.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
HVX45_RS17510	3596046..3597332	-	1287	WP_060765559.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11694.65 Da
• Isoelectric Point: 8.0666

>T165634 WP_032183189.1 NZ_CP057657:3592693-3593007 [Escherichia fergusonii]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSHIRPPERLNPILLLADGKEYVLMTHETATVPVNTLG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T165634 NZ_CP073939:138238-138423 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT165634 WP_000125566.1 NZ_CP057657:3592457-3592690 [Escherichia fergusonii]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT165634 NZ_CP073939:138440-138850 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCAAAAAGATTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCGGAAGCTGCCCGGCGTGTATTGC
CGGGCGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9