Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/FR47-DUF1778
Location 29949..30746 Replicon plasmid pRHB20-C15_2
Accession NZ_CP057633
Organism Citrobacter sp. RHB20-C15

Toxin (Protein)


Gene name KacT Uniprot ID F5S3R9
Locus tag HVX63_RS23515 Protein ID WP_006812498.1
Coordinates 30222..30746 (+) Length 175 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID F5S3R8
Locus tag HVX63_RS23510 Protein ID WP_006812497.1
Coordinates 29949..30218 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVX63_RS23480 25129..26469 - 1341 WP_130097751.1 AAA family ATPase -
HVX63_RS23485 26530..27255 - 726 WP_130097752.1 hypothetical protein -
HVX63_RS23490 27505..27915 - 411 WP_130097753.1 hypothetical protein -
HVX63_RS23495 27905..28549 - 645 WP_130097754.1 hypothetical protein -
HVX63_RS23500 28551..28904 - 354 WP_161215619.1 hypothetical protein -
HVX63_RS23505 28910..29575 - 666 WP_130097756.1 AAA family ATPase -
HVX63_RS23510 29949..30218 + 270 WP_006812497.1 DUF1778 domain-containing protein Antitoxin
HVX63_RS23515 30222..30746 + 525 WP_006812498.1 GNAT family N-acetyltransferase Toxin
HVX63_RS23520 30931..31182 - 252 WP_161215628.1 hypothetical protein -
HVX63_RS23525 31184..31876 - 693 WP_130097758.1 hypothetical protein -
HVX63_RS23530 31891..32214 - 324 WP_130097759.1 hypothetical protein -
HVX63_RS23535 32301..35099 - 2799 WP_130097760.1 tail fiber protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..105909 105909


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-149)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 175 a.a.        Molecular weight: 19356.13 Da        Isoelectric Point: 8.9844

>T165611 WP_006812498.1 NZ_CP057633:30222-30746 [Citrobacter sp. RHB20-C15]
VANLTIEMFSEEAVYDFSCFDCGETSLNDFLNNRLAQQHSGRILRGYLLLTRDPIPKVMGFYTLSGSCFARNTLPSNTQQ
RKVPYSDAPSVTLGRLAIDKSIQRQGYGETLVAHAMKVVYRASLAVGIYALFVDAKNENAMRFYKQLGFTPLTGDNANSL
FYPTKGIEKLFGGK

Download         Length: 525 bp

>T165611 NZ_CP073932:c3904655-3904368 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10114.51 Da        Isoelectric Point: 4.8483

>AT165611 WP_006812497.1 NZ_CP057633:29949-30218 [Citrobacter sp. RHB20-C15]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNIVMDAISNPPAPNDRLKR
AAERLQSME

Download         Length: 270 bp

>AT165611 NZ_CP073932:c3904381-3904079 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0QWY8

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0QUA3

References