Detailed information of TA system TA165610

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4697510..4698138
• Replicon: chromosome
• Accession: NZ_CP057632
• Organism: Citrobacter sp. RHB20-C15

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A7L6Z8W6
• Locus tag: HVX63_RS22460
• Protein ID: WP_044257173.1
• Coordinates: 4697510..4697896 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HVX63_RS22465
• Protein ID: WP_161215612.1
• Coordinates: 4697896..4698138 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVX63_RS22440	4692863..4694200	+	1338	WP_181825192.1	VWA domain-containing protein	-
HVX63_RS22445	4694218..4695714	+	1497	WP_044257176.1	HAMP domain-containing histidine kinase	-
HVX63_RS22450	4695719..4696405	+	687	WP_044257174.1	response regulator transcription factor	-
HVX63_RS22455	4696550..4697479	+	930	WP_042998866.1	formate dehydrogenase accessory protein FdhE	-
HVX63_RS22460	4697510..4697896	-	387	WP_044257173.1	type II toxin-antitoxin system VapC family toxin	Toxin
HVX63_RS22465	4697896..4698138	-	243	WP_161215612.1	ribbon-helix-helix domain-containing protein	Antitoxin
HVX63_RS22470	4698346..4699272	+	927	WP_044257169.1	alpha/beta hydrolase	-
HVX63_RS22475	4699287..4700228	-	942	WP_042998862.1	fatty acid biosynthesis protein FabY	-
HVX63_RS22480	4700273..4700710	-	438	WP_044257167.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HVX63_RS22485	4700707..4701579	-	873	WP_044257165.1	virulence factor BrkB family protein	-
HVX63_RS22490	4701573..4702172	-	600	WP_044257163.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14403.70 Da
• Isoelectric Point: 9.4114

>T165610 WP_044257173.1 NZ_CP057632:c4697896-4697510 [Citrobacter sp. RHB20-C15]
MQKGPVLFDTNILIDLFSGRQEAQQVLEAYPPQNAISLITWMEVMVGAKKYHQEYRTRVALSAFNIIGVSLEIAERSVNL
RQEYGMKLPDAIILATAQIHRFALVTRNTRDFAGIPGVITPYQLQAGR

Nucleotide

• Length: 387 bp

>T165610 NZ_CP073932:3603262-3603540 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9335.53 Da
• Isoelectric Point: 4.2075

>AT165610 WP_161215612.1 NZ_CP057632:c4698138-4697896 [Citrobacter sp. RHB20-C15]
IMAGMDMGRILLDLSDEVIKRLDDLKQQRNLPRAELLREAVEQYLERQGQAETTISNALGLWQGCEEDGVEYQRTLREEW

Nucleotide

• Length: 243 bp

>AT165610 NZ_CP073932:3602999-3603259 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A7L6Z8W6