Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1378019..1378639 Replicon chromosome
Accession NZ_CP057632
Organism Citrobacter sp. RHB20-C15

Toxin (Protein)


Gene name Hha Uniprot ID V1H8E6
Locus tag HVX63_RS06595 Protein ID WP_001280991.1
Coordinates 1378019..1378237 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A3Q8DH98
Locus tag HVX63_RS06600 Protein ID WP_042324455.1
Coordinates 1378265..1378639 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVX63_RS06565 1373672..1374268 + 597 WP_181825317.1 hypothetical protein -
HVX63_RS06570 1374351..1374704 + 354 WP_043001561.1 DUF1428 family protein -
HVX63_RS06575 1374744..1376294 - 1551 WP_181825318.1 EAL domain-containing protein -
HVX63_RS06580 1376517..1376657 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
HVX63_RS06585 1376707..1377177 - 471 WP_043001559.1 YlaC family protein -
HVX63_RS06590 1377291..1377842 - 552 WP_044258934.1 maltose O-acetyltransferase -
HVX63_RS06595 1378019..1378237 - 219 WP_001280991.1 hemolysin expression modulator Hha Toxin
HVX63_RS06600 1378265..1378639 - 375 WP_042324455.1 Hha toxicity modulator TomB Antitoxin
HVX63_RS06605 1379151..1382300 - 3150 WP_043001557.1 multidrug efflux RND transporter permease subunit -
HVX63_RS06610 1382323..1383516 - 1194 WP_043001556.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8613.97 Da        Isoelectric Point: 8.9008

>T165596 WP_001280991.1 NZ_CP057632:c1378237-1378019 [Citrobacter sp. RHB20-C15]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T165596 NZ_CP073932:736473-736769 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14495.31 Da        Isoelectric Point: 4.8989

>AT165596 WP_042324455.1 NZ_CP057632:c1378639-1378265 [Citrobacter sp. RHB20-C15]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT165596 NZ_CP073932:736771-737166 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4ICG

Antitoxin

Source ID Structure
AlphaFold DB A0A3Q8DH98

References