Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/FR47-DUF1778
Location 23313..24110 Replicon plasmid pRHB20-C16_2
Accession NZ_CP057631
Organism Citrobacter sp. RHB20-C16

Toxin (Protein)


Gene name KacT Uniprot ID F5S3R9
Locus tag HVX64_RS23450 Protein ID WP_006812498.1
Coordinates 23313..23837 (-) Length 175 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID F5S3R8
Locus tag HVX64_RS23455 Protein ID WP_006812497.1
Coordinates 23841..24110 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVX64_RS23430 18960..21758 + 2799 WP_130097760.1 tail fiber protein -
HVX64_RS23435 21845..22168 + 324 WP_130097759.1 hypothetical protein -
HVX64_RS23440 22183..22875 + 693 WP_130097758.1 hypothetical protein -
HVX64_RS23445 22877..23128 + 252 WP_161215628.1 hypothetical protein -
HVX64_RS23450 23313..23837 - 525 WP_006812498.1 GNAT family N-acetyltransferase Toxin
HVX64_RS23455 23841..24110 - 270 WP_006812497.1 DUF1778 domain-containing protein Antitoxin
HVX64_RS23460 24484..25149 + 666 WP_130097756.1 AAA family ATPase -
HVX64_RS23465 25155..25508 + 354 WP_161215619.1 hypothetical protein -
HVX64_RS23470 25510..26154 + 645 WP_130097754.1 hypothetical protein -
HVX64_RS23475 26144..26554 + 411 WP_130097753.1 hypothetical protein -
HVX64_RS23480 26804..27529 + 726 WP_130097752.1 hypothetical protein -
HVX64_RS23485 27590..28930 + 1341 WP_130097751.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..105909 105909


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-149)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 175 a.a.        Molecular weight: 19356.13 Da        Isoelectric Point: 8.9844

>T165592 WP_006812498.1 NZ_CP057631:c23837-23313 [Citrobacter sp. RHB20-C16]
VANLTIEMFSEEAVYDFSCFDCGETSLNDFLNNRLAQQHSGRILRGYLLLTRDPIPKVMGFYTLSGSCFARNTLPSNTQQ
RKVPYSDAPSVTLGRLAIDKSIQRQGYGETLVAHAMKVVYRASLAVGIYALFVDAKNENAMRFYKQLGFTPLTGDNANSL
FYPTKGIEKLFGGK

Download         Length: 525 bp

>T165592 NZ_CP073930:c78231-77851 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGCATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACTTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGTGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTTCGGAGTAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10114.51 Da        Isoelectric Point: 4.8483

>AT165592 WP_006812497.1 NZ_CP057631:c24110-23841 [Citrobacter sp. RHB20-C16]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNIVMDAISNPPAPNDRLKR
AAERLQSME

Download         Length: 270 bp

>AT165592 NZ_CP073930:c78452-78231 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGGGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0QWY8

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0QUA3

References