Detailed information of TA system TA165591

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4696439..4697067
• Replicon: chromosome
• Accession: NZ_CP057630
• Organism: Citrobacter sp. RHB20-C16

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A7L6Z8W6
• Locus tag: HVX64_RS22460
• Protein ID: WP_044257173.1
• Coordinates: 4696439..4696825 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HVX64_RS22465
• Protein ID: WP_161215612.1
• Coordinates: 4696825..4697067 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVX64_RS22440	4691792..4693129	+	1338	WP_181825192.1	VWA domain-containing protein	-
HVX64_RS22445	4693147..4694643	+	1497	WP_044257176.1	HAMP domain-containing histidine kinase	-
HVX64_RS22450	4694648..4695334	+	687	WP_044257174.1	response regulator transcription factor	-
HVX64_RS22455	4695479..4696408	+	930	WP_042998866.1	formate dehydrogenase accessory protein FdhE	-
HVX64_RS22460	4696439..4696825	-	387	WP_044257173.1	type II toxin-antitoxin system VapC family toxin	Toxin
HVX64_RS22465	4696825..4697067	-	243	WP_161215612.1	ribbon-helix-helix domain-containing protein	Antitoxin
HVX64_RS22470	4697275..4698201	+	927	WP_044257169.1	alpha/beta hydrolase	-
HVX64_RS22475	4698216..4699157	-	942	WP_042998862.1	fatty acid biosynthesis protein FabY	-
HVX64_RS22480	4699202..4699639	-	438	WP_044257167.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HVX64_RS22485	4699636..4700508	-	873	WP_044257165.1	virulence factor BrkB family protein	-
HVX64_RS22490	4700502..4701101	-	600	WP_044257163.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14403.70 Da
• Isoelectric Point: 9.4114

>T165591 WP_044257173.1 NZ_CP057630:c4696825-4696439 [Citrobacter sp. RHB20-C16]
MQKGPVLFDTNILIDLFSGRQEAQQVLEAYPPQNAISLITWMEVMVGAKKYHQEYRTRVALSAFNIIGVSLEIAERSVNL
RQEYGMKLPDAIILATAQIHRFALVTRNTRDFAGIPGVITPYQLQAGR

Nucleotide

• Length: 387 bp

>T165591 NZ_CP073930:c43509-43222 [Escherichia coli]
ATGACTTATACAGTAAAATTCCGGGATGATGCACTGAAAGAATGGCTCAGACTGGACAAGACCATTCAGCAGCAGTTCGC
AAAAAAACTGAAAAAATGCAGTGAAAATCCTCATATAGCTTCTGCAAAACTACGTGGATTAAAGGACTGCTATAAAATCA
AACTTCGCGCATCAGGTTTTCGTCTGGTTTATCAGATTATTGATGATATGTTAATTATTGCGGTCGTCGCAGTAGGTAAA
CGTGAACGCAGCAACGTTTATCATCTCGCCAGTGAACGGATGAAATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9335.53 Da
• Isoelectric Point: 4.2075

>AT165591 WP_161215612.1 NZ_CP057630:c4697067-4696825 [Citrobacter sp. RHB20-C16]
IMAGMDMGRILLDLSDEVIKRLDDLKQQRNLPRAELLREAVEQYLERQGQAETTISNALGLWQGCEEDGVEYQRTLREEW

Nucleotide

• Length: 243 bp

>AT165591 NZ_CP073930:c43757-43506 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAGCTGAAAAAAAATCCGATGGCAACTGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCAGCATTCTACTGTGTCCCTGCTGAGCTCTATGAAAGAATGC
TTGATGCCTTGGATGACCAGGAGCTGGTAAAACTGGTTACTGAACGCAGCAGTCAGCCTCTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Structures

Toxin

• AlphaFold DB: A0A7L6Z8W6