Detailed information of TA system TA165589

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4125192..4125895
• Replicon: chromosome
• Accession: NZ_CP057630
• Organism: Citrobacter sp. RHB20-C16

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: HVX64_RS19765
• Protein ID: WP_181825121.1
• Coordinates: 4125192..4125533 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HVX64_RS19770
• Protein ID: WP_181825122.1
• Coordinates: 4125554..4125895 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVX64_RS19745	4120393..4121268	+	876	WP_000774153.1	3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase	-
HVX64_RS19750	4121317..4121607	+	291	WP_042999272.1	(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase	-
HVX64_RS19755	4121618..4122364	+	747	WP_044256838.1	epimerase	-
HVX64_RS19760	4122967..4125000	+	2034	WP_181825120.1	hypothetical protein	-
HVX64_RS19765	4125192..4125533	-	342	WP_181825121.1	TA system toxin CbtA family protein	Toxin
HVX64_RS19770	4125554..4125895	-	342	WP_181825122.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HVX64_RS19775	4125906..4126448	-	543	WP_181825123.1	DNA repair protein RadC	-
HVX64_RS19780	4126461..4126919	-	459	WP_181825124.1	antirestriction protein	-
HVX64_RS19785	4127005..4127241	-	237	WP_181825125.1	DUF905 domain-containing protein	-
HVX64_RS19790	4127340..4128248	-	909	WP_181825126.1	hypothetical protein	-
HVX64_RS19795	4128260..4128484	-	225	WP_181825127.1	DUF4339 domain-containing protein	-
HVX64_RS19800	4128522..4129220	-	699	WP_181825128.1	DeoR family transcriptional regulator	-
HVX64_RS19805	4129429..4130253	-	825	WP_181825129.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4122967..4156605	33638

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12804.74 Da
• Isoelectric Point: 9.6552

>T165589 WP_181825121.1 NZ_CP057630:c4125533-4125192 [Citrobacter sp. RHB20-C16]
MKTLPATTPQAVTLCQSSVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRKGF
NWQEHSPYLRAVDILRARQATGLLRRSRKNAVR

Nucleotide

• Length: 342 bp

>T165589 NZ_CP073929:c4100276-4099989 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGTATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12739.23 Da
• Isoelectric Point: 5.9564

>AT165589 WP_181825122.1 NZ_CP057630:c4125895-4125554 [Citrobacter sp. RHB20-C16]
MKSTGSENDSFLQWGLNRNVTPCFGARLVQEGNRLHYLVDRASITGEFSNAESLKLDEVFPHFISQMESMLTTGEVNPRH
AHCVTLYHNGFTCEADTLGSYGYVYIAVYPTQR

Nucleotide

• Length: 342 bp

>AT165589 NZ_CP073929:c4100002-4099700 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA